Rabbit SOCS2 Antibody | anti-SOCS2 antibody

Rabbit anti SOCS-2 antibody

Cat. Num. AAA462132

• Gene Names: SOCS2; CIS2; SSI2; Cish2; SSI-2; SOCS-2; STATI2
• Reactivity: Human, Mouse, Rat, Chicken, Bovine, Dog
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: The Rabbit IgG is purified by Epitope Affinity Purification.
• Synonyms: SOCS2; Rabbit anti SOCS-2 antibody; Rabbit anti-SOCS-2; Suppressor of Cytokine Signaling 2 (SOCS2);; anti-SOCS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Chicken, Bovine, Dog
• Specificity: This antibody recognizes ~23 kDa of SOCS-2 protein from human and mouse origins. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer.
• Concentration: Size: 100 ug/200 ul (varies by lot)

• Applicable Applications for anti-SOCS2 antibody: ELISA, Western Blot, Immunohistochemistry
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 0.5-2 ug/ml
• Antigen Preparation: A synthetic peptide corresponding to the C-terminus of SOCS-2 protein from human, mouse and rat origins.
• Positive Control: Mouse muscle lysate
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Testing Data

Related Product Information for anti-SOCS2 antibody

Cytokines induce a variety of biological responses by binding to specific cell surface receptors and activating cytoplasmic signal transduction pathways which are suppressed by other transcriptional factors. Proteins that mediate these signals from the cell surface to the nucleus include JAKs and STATs, while SOCS (Suppressor of cytokine signaling) proteins inhibit those signaling. The SOCS family comprises at least 8 members, SOCS1 to SOCS7 and CIS (cytokine inducible SH2 containing protein). Structurally, the SOCS proteins are composed of an N terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C terminal motif named the SOCS box. SOCS 2 is considered to regulate postnatal growth, such as GH/IGF-1 pathway.

References

Bhakta R. Dey, et al. Interaction of Human Suppressor of Cytokine Signaling (SOCS)-2 with the Insulin-like Growth Factor-I Receptor J. Biol. Chem., Sep 1998; 273: 24095 - 24101

NCBI and Uniprot Product Information

• NCBI GI #: 4507263
• NCBI GeneID: 8835
• NCBI Accession #: NP_003868.1
• NCBI GenBank Nucleotide #: NM_003877.3
• UniProt Accession #: O14508; O14542; O95102; Q9UKS5
• Molecular Weight: 23 kDa
• NCBI Official Full Name: suppressor of cytokine signaling 2
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 2
• NCBI Official Symbol: SOCS2
• NCBI Official Synonym Symbols: CIS2; SSI2; Cish2; SSI-2; SOCS-2; STATI2
• NCBI Protein Information: suppressor of cytokine signaling 2; CIS-2; STAT induced STAT inhibitor-2; STAT-induced STAT inhibitor 2; STAT-induced STAT inhibitor-2; cytokine-inducible SH2 protein 2; suppressor of cytokine signaling-2
• UniProt Protein Name: Suppressor of cytokine signaling 2
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS2
• UniProt Synonym Gene Names: CIS2; SSI2; STATI2
• UniProt Entry Name: SOCS2_HUMAN

NCBI Description

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10 and interferon (IFN)-gamma. The protein encoded by this gene is found to interact with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R), and thus is thought to be involved in the regulation of IGF1R mediated cell signaling. Knockout studies in mice also suggested a regulatory role of this gene in IGF-1 related growth control. [provided by RefSeq]

Uniprot Description

Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with IGF1 receptor, prolactin receptor and growth hormone (GH) receptor. Associates with the Elongin BC complex.

Tissue specificity: High expression in heart, placenta, lung, kidney and prostate.

Induction: By a subset of cytokines, including EPO/erythropoietin and CSF2/GM-CSF.

Domain: The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.

Sequence similarities: Contains 1 SH2 domain.Contains 1 SOCS box domain.