Testing Data
Rabbit AKT1/PKBa Antibody | anti-AKT1/PKBa antibody
Rabbit anti AKT1/PKBa antibody
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope affinity purification
Synonyms
AKT1/PKBa; Rabbit anti AKT1/PKBa antibody; Rabbit anti-AKT1; anti-AKT1/PKBa antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Specificity
This antibody only recognizes ~60 kDa of human AKT1, not AKT1 or AKT2. It also reacts with mouse and rat AKT3 protein. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope affinity purification
Form/Format
This affinity purified antibody is supplied in sterile phosphate buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-AKT1/PKBa antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the close to C-term of human AKT1 protein.
Positive Control
Hela lysate
Cellular Location
Cytoplasmic
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-AKT1/PKBa antibody
Akt [also known as PKB (Protein kinase B) is a family of serine/threonine kinases that plays an important role in signal transduction. There are three known isoforms of Akt in mammalian cells [Akt1 (alpha), Akt2 (beta) and Akt3 (gamma)]; Akt2 and Akt3 have 81% and 83% homology in amino acid sequences with Akt1, respectively. Numerous studies have revealed that the expression pattern of Akt1 and Akt2 is ubiquitous, whereas Akt3 is expressed predominantly in brain, heart, and kidney. Akt is activated by insulin and growth and survival factors.Akt has been referred to as an oncogene because it has increased activity in a number of tumors.
References
Kaname Nakatani, et al. Up-regulation of Akt3 in Estrogen Receptor-deficient Breast Cancers and Androgen-independent Prostate Cancer Lines. THE JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 274, No. 31, Issue of July 30, pp. 21528-21532, 1999.
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Product Notes
The AKT1/PKBa (Catalog #AAA462004) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti AKT1/PKBa antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AKT1/PKBa can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 0.5-2 ug/ml. Researchers should empirically determine the suitability of the AKT1/PKBa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT1/PKBa, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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