Mouse anti-Human HCV Envelope Antigen Monoclonal Antibody
Mouse Anti HCV Envelope Antigen Monoclonal Antibody
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Protein A affinity purification eluted according to the isotyping IgG1.kappa.
Synonyms
HCV Envelope Antigen; Monoclonal Antibody; Mouse Anti HCV Envelope Antigen Monoclonal Antibody; Hepatitis C Virus; HCVgp1; Mouse anti-HCV NS3/NS4; anti-HCV Envelope Antigen antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1.kappa
Clone Number
ABM111
Specificity
HCV NS3/NS4 antigen
Purity/Purification
Protein A affinity purification eluted according to the isotyping IgG1.kappa.
Applicable Applications for anti-HCV Envelope Antigen antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Affinicity Constant
2x109 L/Mol
Immunogen
Recombinant HCV NS3, NS4 antigen
Lymphocyte Strain
Babl/c mouse
Myeloma
P3x63 Ag 8.653
Immunization Procedure
100ul of emulsion, mixed with equal volume of recombinant HCV solution and Freund complete adjuvant is injected by using the intracutaneous injection method to each balb/c mouse, followed by boost-inject every 2 to 3 weeks with emulsion; the mice of high titer of antibody yield will be chosen for the fusion procedure.
Fusion Procedure
Fusion was performed by addition of chemical fusion reagent PEG to the mixture of spleen cells and myeloma cells at a ratio 5:1; Seeding the cell suspension to 96 well cell culture plates and selected according to principle of HAT drug blocking the de novo synthesis of nucleotides.
Screening Method
EIA method is used for clone screening, in which HCV NS3&NS4 antigen is labeled with HRP for the screening of positive clones.
Target Antigen
HCV NS3/NS4: Purity>90%
Hybridoma Cloning History
The clone was screened from a total of four 96 well cell culture plate and was subcloned twice by using EIA method. Both supernatant and purified IgG from ascites were evaluated by the method of EIA. Further evaluation was accomplished by applying purified IgG to the EIA test against normal human sera and HCV positive sera specimens; no cross reaction was found with HBeAg, HBsAg, HBcAg, AFP.
References
Masahiko Kaito, et al. Hepatitis C virus particle detected by immunoelectron microscopic study. Journal of General Virology. (1994), 75, 1735-1760. Saleh MG, Pereira LM, Tibbs CJ, Ziu M, Al-Fituri MO, Williams R, McFarlane IG. High prevalence of hepatitis C virus in the normal Libyan population. Trans R Soc Trop Med Hyg (1994), 88(3): 292-4.
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Product Notes
The HCV Envelope Antigen (Catalog #AAA460740) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti HCV Envelope Antigen Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HCV Envelope Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the HCV Envelope Antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCV Envelope Antigen, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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