Rabbit AEG1 Polyclonal Antibody | anti-AEG1 antibody

Rabbit anti AEG1 (C-Term) Polyclonal Antibody

Cat. Num. AAA460568

• Gene Names: MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: AEG1; Polyclonal Antibody; Rabbit anti AEG1 (C-Term) Polyclonal Antibody; Metadherin; 3D3; AEG-1; LYRIC; LYRIC/3D3; anti-AEG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes ~64 kDa of human AEG1 protein. It also reacts with human, mouse and rat origins. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 582

• Applicable Applications for anti-AEG1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide derived from the c-term of human AEG1. This sequence is identical to human, mouse, rat and bovine
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-AEG1 antibody

AEG1 (Astrocyte elevated gene 1), also called Metadherin (MTDH), or L LYsine-RIch CEACAM1 co-isolated (LYRIC), is 582 aa single transmembrane domain (TMD) protein, this protein is widely expressed and highly conserved between species. It is not a structural component for tight junctional (TJ) complexes, it is recruited during the maturation of the tight junction complex and co-localizes with tight junction proteins ZO-1 and occludin in polarized epithelial cells. This novel protein is also reported as an HIV-1 inducible gene, which is expressed ubiquitously with higher expression in tissues containing muscular actin and its expression is increased in astrocytes infected with HIV-1 or treated with gp120 or tumor necrosis factor (TNF)-alpha. AEG1 expression is elevated in subsets of breast carcinomas, malignant gliomas and melanomas. AEG1 may affect tumor progression in multiple cell lineages by augmenting expression of the transformed phenotype and/or by inducing glutamate excitotoxicity in malignant glioma. In these contexts, an HIV-1-inducible gene, AEG-1, may contribute to multiple brain abnormalities and tumor formation, by both common and distinct mechanisms.

References

Sutherland HG, et al. 3D3/lyric: a novel transmembrane protein of the endoplasmic reticulum and nuclear envelope, which is also present in the nucleolus. Exp Cell Res. 2004 Mar 10;294 (1):94-105.

NCBI and Uniprot Product Information

• NCBI GI #: 29122959
• NCBI GeneID: 92140
• Molecular Weight: 63,837 Da
• NCBI Official Full Name: astrocyte elevated gene-1 AEG1
• NCBI Official Synonym Full Names: metadherin
• NCBI Official Symbol: MTDH
• NCBI Official Synonym Symbols: 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
• NCBI Protein Information: protein LYRIC
• UniProt Protein Name: Protein LYRIC
• UniProt Gene Name: MTDH
• UniProt Synonym Gene Names: AEG1; LYRIC; AEG-1

Uniprot Description

Lyric: a protein of unknown function.

Protein type: Cell adhesion; Membrane protein, integral; Nucleolus

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: apical plasma membrane; cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; fibrillar center; intercellular canaliculus; nuclear body; nucleus; perinuclear region of cytoplasm; tight junction

Molecular Function: double-stranded RNA binding; NF-kappaB binding; protein binding; RNA binding; transcription coactivator activity

Biological Process: activation of NF-kappaB transcription factor; lipopolysaccharide-mediated signaling pathway; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein kinase B signaling cascade