Rabbit anti-Human CDC25b Polyclonal Antibody | anti-CDC25b antibody
Rabbit anti CDC25b Polyclonal Antibody
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Epitope Affinity Purification
Synonyms
CDC25b; Polyclonal Antibody; Rabbit anti CDC25b Polyclonal Antibody; Cell Division cycle 25b; anti-CDC25b antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes human CDC25B protein. The other species were not tested.
Purity/Purification
Purified by Epitope Affinity Purification
Form/Format
Supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-CDC25b antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ELISA: 0.01-0.1 ug/ml
Flow Cytometry: 5-10 ug/ml
Immunohistochemistry: 2-10 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml
Flow Cytometry: 5-10 ug/ml
Immunohistochemistry: 2-10 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml
Preparation
A synthetic peptide derived from N-terminus of human CDC25B protein.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Related Product Information for anti-CDC25b antibody
Three homologues of CDC25 called CDC25A/B/C Cdc25 tyrosine phosphatases positively regulate the cell cycle by activating cyclin-dependent kinase/cyclin complexes CDK1 (CDC2). Dephosphorylation of threonine 14 and tyrosine 15 of CDK1 by CDC25 phosphatases is a key step in the activation of the CDK1-cycline B protein kinase. Of the three mammalian Cdc25 isoforms, Cdc25A is phosphorylated by genotoxic stress-activated Chk1 or Chk2, which triggers its SCFbeta-TrCP-mediated degradation. CDC25B is degraded by the proteasome. CDK1-cyclin A phosphorylation targets CDC25B for degradation and this might be an important component of cell cycle regulation at the G2/M transition.
References
Véronique Baldin, et al, Phosphorylation of Human CDC25B Phosphatase by CDK1-Cyclin A Triggers Its Proteasome-dependent Degradation. The Journal of Biological Chemistry, 272, 32731-32734 (1997).
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Product Notes
The CDC25b (Catalog #AAA460389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CDC25b Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC25b can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). ELISA: 0.01-0.1 ug/ml Flow Cytometry: 5-10 ug/ml Immunohistochemistry: 2-10 ug/ml Immunoprecipitation: 2-5 ug/ml Western Blot: 0.1-1 ug/ml. Researchers should empirically determine the suitability of the CDC25b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC25b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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