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Western Blot (WB) (The whole cell lysate derived from mouse brain tissue was immunoblotted Rabbit anti-TAFA2 (Cat# MBS460333) at 1:500. An immunoreactive band band around 12 kDa was observed.)

Rabbit anti-Human, Mouse TAFA2 Polyclonal Antibody | anti-TAFA2 antibody

Rabbit anti TAFA2 Polyclonal Antibody

Gene Names
FAM19A2; TAFA2; TAFA-2
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Epitope Affinity Purification.
Synonyms
TAFA2; Polyclonal Antibody; Rabbit anti TAFA2 Polyclonal Antibody; family with sequence similarity 19 (chemokine (C-C motif)-like); member A2; FATA-2; FAM19A2; anti-TAFA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes TAFA2 protein. It reacts to human, mice, and rat.
Purity/Purification
Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Sequence Length
131
Applicable Applications for anti-TAFA2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
EIA: 0.01-0.1 ug/ml
IHC: 2-10 ug/ml
IP: 2-5 ug/ml
WB: 0.1-1 ug/ml
Optimal dilutions should be determined by the researchers for the specific applications.
Antigen Preperation
A synthetic peptide corresponding to the N-terminus of TAFA2 protein. This sequence is identical among human, mouse.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at –20°C to –70°C.
The antibodies can be stored at 2°C to 8°C for three month without detectable loss of activity.
Avoid repeated freezing-thawing cycles.

Western Blot (WB)

(The whole cell lysate derived from mouse brain tissue was immunoblotted Rabbit anti-TAFA2 (Cat# MBS460333) at 1:500. An immunoreactive band band around 12 kDa was observed.)

Western Blot (WB) (The whole cell lysate derived from mouse brain tissue was immunoblotted Rabbit anti-TAFA2 (Cat# MBS460333) at 1:500. An immunoreactive band band around 12 kDa was observed.)

Immunohistochemistry (IHC)

(Human brain tissue was immune-stained with Anti-TAFA2 antibody, (Cat# MBS460333) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

Immunohistochemistry (IHC) (Human brain tissue was immune-stained with Anti-TAFA2 antibody, (Cat# MBS460333) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

Immunohistochemistry (IHC)

(Immunohistochemistry: Human tonsil tissue (FFPE)was immune-stained with Anti-TAFA2 antibody, (Cat#MBS460333) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)

Immunohistochemistry (IHC) (Immunohistochemistry: Human tonsil tissue (FFPE)was immune-stained with Anti-TAFA2 antibody, (Cat#MBS460333) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Related Product Information for anti-TAFA2 antibody
Background: The TAFA2, also known as FAM19A2, is a secreted 12kDa protein. It belongs to the FAM19/TAFA family of chemokinelike. The family is composed of five highly homologous genes referred to as TAFA-1 to -5. The TAFA genes encode proteins of approximately 100 amino acids that contain conserved cysteine residues at fixed positions. TAFA-1 to -4 are more closely related to each other than to TAFA-5, in which a conserved motif including CC in TAFA-1 to -4 is not present. In H. sapiens, TAFA-3 has two isoforms formed by alternative splicing. TAFAs are highly expressed in central nervous system (CNS), and less extent in other tissues such as colon, heart, lung, spleen, kidney, and thymus. TAFA2 is expression is highest in the occipital and frontal cortex. TAFAs may modulate immune responses in the CNS by functioning as brainspecific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS. It may act as neurokines that regulates immune nervous cells . It may also control axonal sprouting following brain injury

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
12.0
NCBI Official Full Name
TAFA2
NCBI Official Synonym Full Names
family with sequence similarity 19 member A2, C-C motif chemokine like
NCBI Official Symbol
FAM19A2
NCBI Official Synonym Symbols
TAFA2; TAFA-2
NCBI Protein Information
protein FAM19A2
UniProt Protein Name
Protein FAM19A2
UniProt Gene Name
FAM19A2
UniProt Synonym Gene Names
TAFA2

NCBI Description

This gene is a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins. These proteins contain conserved cysteine residues at fixed positions, and are distantly related to MIP-1alpha, a member of the CC-chemokine family. The TAFA proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines, that act as regulators of immune and nervous cells. [provided by RefSeq, Jul 2008]

Uniprot Description

FAM19A2: a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins. These proteins contain conserved cysteine residues at fixed positions, and are distantly related to MIP-1alpha, a member of the CC-chemokine family. The TAFA proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines, that act as regulators of immune and nervous cells. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q14.1

Research Articles on TAFA2

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Product Notes

The TAFA2 fam19a2 (Catalog #AAA460333) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti TAFA2 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TAFA2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). EIA: 0.01-0.1 ug/ml IHC: 2-10 ug/ml IP: 2-5 ug/ml WB: 0.1-1 ug/ml Optimal dilutions should be determined by the researchers for the specific applications. Researchers should empirically determine the suitability of the TAFA2 fam19a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAFA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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