Human Glutathione Peroxidase 4 (GPX4) RTU ELISA Kit | GPX4 rtu elisa kit

Human Glutathione Peroxidase 4 (GPX4) ELISA Kit

Cat. Num. AAA459694

• Gene Names: GPX4; MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
• Reactivity: Human
• Synonyms: Glutathione Peroxidase 4 (GPX4); Human Glutathione Peroxidase 4 (GPX4) ELISA Kit; MCSP; PHGPx; SnGPx; SnPHGPx; Phospholipid Hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; mitochondrial; GPX4 rtu elisa kit

• Reactivity: Human
• Sequence Length: 227

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPX4 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPX4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GPX4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPX4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GPX4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 90903238
• NCBI GeneID: 2879
• NCBI Accession #: NP_001034936.1
• NCBI GenBank Nucleotide #: NM_001039847.2
• UniProt Accession #: P36969; O43381; Q6PJ59; Q9UPK2
• Molecular Weight: 19,525 Da
• NCBI Official Full Name: phospholipid hydroperoxide glutathione peroxidase isoform B
• NCBI Official Synonym Full Names: glutathione peroxidase 4
• NCBI Official Symbol: GPX4
• NCBI Official Synonym Symbols: MCSP; SMDS; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
• NCBI Protein Information: phospholipid hydroperoxide glutathione peroxidase
• UniProt Protein Name: Phospholipid hydroperoxide glutathione peroxidase
• Protein Family: Probable glutathione peroxidase
• UniProt Gene Name: GPX4
• UniProt Synonym Gene Names: GSHPx-4

NCBI Description

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016]

Uniprot Description

Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (). Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide (). Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (). Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species (PubMed:24439385). The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis (). The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (). May be required to protect cells from the toxicity of ingested lipid hydroperoxides (). Required for normal sperm development and male fertility (). Essential for maturation and survival of photoreceptor cells (). Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion ().