Mouse E1A Binding Protein P300 (EP300) RTU ELISA Kit | EP300 rtu elisa kit

Mouse E1A Binding Protein P300 (EP300) ELISA Kit

Cat. Num. AAA459070

• Gene Names: Ep300; p300; KAT3B; p300 HAT; A430090G16; A730011L11
• Reactivity: Mouse
• Synonyms: E1A Binding Protein P300 (EP300); Mouse E1A Binding Protein P300 (EP300) ELISA Kit; KAT3B; p300; Histone Acetyltransferase p300; EP300 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 2412

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.051ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EP300 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EP300. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EP300. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EP300, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EP300 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 94421034
• NCBI GeneID: 328572
• NCBI Accession #: NP_808489.4
• NCBI GenBank Nucleotide #: NM_177821.6
• UniProt Accession #: B2RWS6; E9PYJ8
• Molecular Weight: 263,305 Da
• NCBI Official Full Name: histone acetyltransferase p300
• NCBI Official Synonym Full Names: E1A binding protein p300
• NCBI Official Symbol: Ep300
• NCBI Official Synonym Symbols: p300; KAT3B; p300 HAT; A430090G16; A730011L11
• NCBI Protein Information: histone acetyltransferase p300
• UniProt Protein Name: Histone acetyltransferase p300
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: Ep300
• UniProt Synonym Gene Names: P300; p300 HAT

Uniprot Description

Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (). Acetylates all four core histones in nucleosomes (). Histone acetylation gives an epigenetic tag for transcriptional activation (). Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein (PubMed:18486321, PubMed:24216764). Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (). Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (). Also functions as acetyltransferase for nonhistone targets (). Acetylates 'Lys-131' of ALX1 and acts as its coactivator (). Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (). Acetylates HDAC1 leading to its inactivation and modulation of transcription (). Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (). Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium (). Promotes cardiac myocyte enlargement (). Can also mediate transcriptional repression (). Acetylates FOXO1 and enhances its transcriptional activity (). Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (). Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (). Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:14645221, PubMed:9512516). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (). Acetylates MEF2D (). Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degragation, this mechanism may be involved in CD4/CD8 lineage differentiation (PubMed:20810990). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA) or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation or propionylation, respectively (PubMed:27105113). Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (E)-but-2-enoyl-CoA (crotonyl-CoA) concentration is low (). Also acts as a histone butyryltransferase; butyrylation marks active promoters (PubMed:27105113).