Mouse Crystallin Alpha B (CRYaB) RTU ELISA Kit | CRYaB rtu elisa kit

Mouse Crystallin Alpha B (CRYaB) ELISA Kit

Cat. Num. AAA458602

• Gene Names: Cryab; P23; Crya2; HspB5; Crya-2
• Reactivity: Mouse
• Synonyms: Crystallin Alpha B (CRYaB); Mouse Crystallin Alpha B (CRYaB) ELISA Kit; CRY-AB; CRYA-B; CRYA2; CTPP2; HSPB5; Heat shock protein beta-5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component; CRYaB rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 175

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRYaB rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRYaB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CRYaB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRYaB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CRYaB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 577019561
• NCBI GeneID: 12955
• NCBI Accession #: NP_001276711.1
• NCBI GenBank Nucleotide #: NM_001289782.1
• UniProt Accession #: P23927; Q64325
• Molecular Weight: 20,069 Da
• NCBI Official Full Name: alpha-crystallin B chain
• NCBI Official Synonym Full Names: crystallin, alpha B
• NCBI Official Symbol: Cryab
• NCBI Official Synonym Symbols: P23; Crya2; HspB5; Crya-2
• NCBI Protein Information: alpha-crystallin B chain
• UniProt Protein Name: Alpha-crystallin B chain
• Protein Family: Alpha-crystallin
• UniProt Gene Name: Cryab
• UniProt Synonym Gene Names: Crya2

NCBI Description

This gene encodes a member of the small heat-shock protein (HSP20) family. The encoded protein is a molecular chaperone that protects proteins against thermal denaturation and other stresses. This protein is a component of the eye lens, regulates lens differentiation and functions as a refractive element in the lens. This protein is a negative regulator of inflammation, has anti-apoptotic properties and also plays a role in the formation of muscular tissue. Mice lacking this gene exhibit worse experimental autoimmune encephalomyelitis and inflammation of the central nervous system compared to the wild type. In mouse models, this gene has a critical role in alleviating the pathology of the neurodegenerative Alexander disease. Mutations in the human gene are associated with myofibrillar myopathy 2, fatal infantile hypertonic myofibrillar myopathy, multiple types of cataract and dilated cardiomyopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.