Mouse Corticotropin Releasing Hormone (CRH) RTU ELISA Kit | CRH rtu elisa kit

Mouse Corticotropin Releasing Hormone (CRH) ELISA Kit

Cat. Num. AAA458565

• Gene Names: Crh; CRF; Gm1347
• Reactivity: Mouse
• Synonyms: Corticotropin Releasing Hormone (CRH); Mouse Corticotropin Releasing Hormone (CRH) ELISA Kit; CRF; Corticotropin Releasing Factor; Corticoliberin; CRH rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 187

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 4.45pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Related Product Information for CRH rtu elisa kit

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to CRH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CRH and unlabeled CRH (Standards or samples) with the pre-coated antibody specific to CRH. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CRH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CRH in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 45429990
• NCBI GeneID: 12918
• NCBI Accession #: NP_991338.1
• NCBI GenBank Nucleotide #: NM_205769.3
• UniProt Accession #: Q8CIT0; Q14AA2
• Molecular Weight: 20,778 Da
• NCBI Official Full Name: corticoliberin preproprotein
• NCBI Official Synonym Full Names: corticotropin releasing hormone
• NCBI Official Symbol: Crh
• NCBI Official Synonym Symbols: CRF; Gm1347
• NCBI Protein Information: corticoliberin
• UniProt Protein Name: Corticoliberin
• Protein Family: Corticoliberin
• UniProt Gene Name: Crh
• UniProt Synonym Gene Names: Gm1347; CRF

NCBI Description

This gene encodes a member of the corticotropin-releasing factor family and preproprotein that is proteolytically processed to generate a mature protein product. This protein product is a neuropeptide hormone that binds to the corticotropin releasing hormone receptors (CRHR1 and CRHR2) to stimulate the release of adrenocorticotropic hormone from the pituitary gland in response to stress. The encoded protein may also regulate angiogenesis and inflammation. Homozygous knockout mice for this gene exhibit reduced corticosterone levels while the offspring of these mice die perinatally. [provided by RefSeq, Aug 2015]

Uniprot Description

Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (PubMed:23470617).