Mouse Apelin (APLN) RTU ELISA Kit | APLN rtu elisa kit

Mouse Apelin (APLN) ELISA Kit

Cat. Num. AAA457678

• Gene Names: Apln; Apel; 6030430G11Rik
• Reactivity: Mouse
• Synonyms: Apelin (APLN); Mouse Apelin (APLN) ELISA Kit; XNPEP2; APEL; APJ endogenous ligand; APLN rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 77

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 125-8000pg/mL
• Sensitivity: 37.5pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Related Product Information for APLN rtu elisa kit

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to APLN has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled APLN and unlabeled APLN (Standards or samples) with the pre-coated antibody specific to APLN. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of APLN in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of APLN in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 7304895
• NCBI GeneID: 30878
• NCBI Accession #: NP_038940.1
• NCBI GenBank Nucleotide #: NM_013912.4
• UniProt Accession #: Q9R0R4
• Molecular Weight: 8,658 Da
• NCBI Official Full Name: apelin preproprotein
• NCBI Official Synonym Full Names: apelin
• NCBI Official Symbol: Apln
• NCBI Official Synonym Symbols: Apel; 6030430G11Rik
• NCBI Protein Information: apelin
• UniProt Protein Name: Apelin
• Protein Family: Apelin
• UniProt Gene Name: Apln
• UniProt Synonym Gene Names: Apel

NCBI Description

This gene encodes the neuropeptide Apelin, an endogenous ligand of the G protein-coupled receptor APJ. This gene is highly expressed in the central nervous system and peripheral tissues and the encoded preproprotein undergoes proteolytic processing to generate mature peptides of different sizes that are secreted into the plasma. These peptides play important roles in a broad range of physiological processes such as cardiac contractility, blood pressure, blood vessel growth, appetite and drink behavior, and pituitary hormone secretion. Mice lacking the encoded protein develop progressive heart failure and exhibit a shorter bleeding time, prothrombotic profile and increased bone mass. [provided by RefSeq, Jul 2016]

Uniprot Description

Endogenous ligand for the apelin receptor (APLNR). Drives internalization of APLNR (). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (PubMed:10525157). Plays a role in early coronary blood vessels formation (PubMed:28890073). Mediates myocardial contractility in an ERK1/2-dependent manner (). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (PubMed:11359874).