Human Alcohol Dehydrogenase 1 (ADH1) RTU ELISA Kit | ADH1 rtu elisa kit

Human Alcohol Dehydrogenase 1 (ADH1) ELISA Kit

Cat. Num. AAA457402

• Gene Names: ADH1A; ADH1
• Reactivity: Human
• Synonyms: Alcohol Dehydrogenase 1 (ADH1); Human Alcohol Dehydrogenase 1 (ADH1) ELISA Kit; ADH1A; Alcohol Dehydrogenase 1A(Class I)Alpha Polypeptide; Alcohol dehydrogenase subunit alpha; ADH1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 375

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.72ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADH1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADH1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4501929
• NCBI GeneID: 124
• NCBI Accession #: NP_000658.1
• NCBI GenBank Nucleotide #: NM_000667.3
• UniProt Accession #: P07327; Q17R68; A8K3E3
• Molecular Weight: 39,859 Da
• NCBI Official Full Name: alcohol dehydrogenase 1A
• NCBI Official Synonym Full Names: alcohol dehydrogenase 1A (class I), alpha polypeptide
• NCBI Official Symbol: ADH1A
• NCBI Official Synonym Symbols: ADH1
• NCBI Protein Information: alcohol dehydrogenase 1A
• UniProt Protein Name: Alcohol dehydrogenase 1A
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH1A
• UniProt Synonym Gene Names: ADH1

NCBI Description

This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Uniprot Description

MiscellaneousThere are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.