Rat A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) RTU ELISA Kit | ADAMTS1 rtu elisa kit

Rat A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) ELISA Kit

Cat. Num. AAA457385

• Reactivity: Rat
• Synonyms: A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1); Rat A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1) ELISA Kit; C3-C5; METH1; ADAMTS1 rtu elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADAMTS1. No significant cross-reactivity or interference between ADAMTS1 and analogues was observed.
• Sequence Length: 967

• Samples: Rat serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: <30.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADAMTS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADAMTS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS1 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ADAMTS1 in rat serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAMTS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADAMTS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADAMTS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADAMTS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148747506
• NCBI GeneID: 79252
• NCBI Accession #: NP_077376.2
• NCBI GenBank Nucleotide #: NM_024400.2
• UniProt Accession #: Q68EJ2; Q9WUQ1
• Molecular Weight: 105,648 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 1
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 1
• NCBI Official Symbol: Adamts1
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 1
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 1
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: Adamts1

NCBI Description

disintegrin and metalloprotease that may be necessary for normal kidney morphology and function [RGD, Feb 2006]

Uniprot Description

CautionLacks conserved residue(s) required for the propagation of feature annotation.