Mouse Surfactant Associated Protein C (SPC) ELISA Kit | SPC elisa kit

Mouse Surfactant Associated Protein C (SPC) ELISA Kit

Cat. Num. AAA457188

• Gene Names: Sftpc; SP5; SPC; SP-C; Sftp2; Bricd6; Sftp-2; pro-SpC
• Reactivity: Mouse
• Synonyms: Surfactant Associated Protein C (SPC); Mouse Surfactant Associated Protein C (SPC) ELISA Kit; SFTPC; PSP-C; SFTP2; SP-C; SP5; Pulmonary Surfactant Protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SPC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of SPC. No significant cross-reactivity or interference between SPC and analogues was observed.
• Sequence Length: 193

• Samples: Serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: <0.216ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SPC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SPC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SPC in mouse serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SPC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 131426
• NCBI GeneID: 20389
• UniProt Accession #: P21841
• Molecular Weight: 21,055 Da
• NCBI Official Full Name: Pulmonary surfactant-associated protein C
• NCBI Official Synonym Full Names: surfactant associated protein C
• NCBI Official Symbol: Sftpc
• NCBI Official Synonym Symbols: SP5; SPC; SP-C; Sftp2; Bricd6; Sftp-2; pro-SpC
• NCBI Protein Information: pulmonary surfactant-associated protein C
• UniProt Protein Name: Pulmonary surfactant-associated protein C
• UniProt Gene Name: Sftpc
• UniProt Synonym Gene Names: Sftp2; SP-C

Uniprot Description

Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.