Human Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) ELISA Kit | MAP1LC3b elisa kit

Human Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) ELISA Kit

Cat. Num. AAA457076

• Gene Names: MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• Reactivity: Human
• Synonyms: Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b); Human Microtubule Associated Protein 1 Light Chain 3 Beta (MAP1LC3b) ELISA Kit; MAP1A/1BLC3; ATG8F; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1LC3b elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MAGEA4. No significant cross-reactivity or interference between MAGEA4 and analogues was observed.
• Sequence Length: 125

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058g/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MAGEA4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MAGEA4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAP1LC3b elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MAGEA4 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAGEA4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MAGEA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAGEA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MAGEA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 12383056
• NCBI GeneID: 81631
• NCBI Accession #: NP_073729.1
• NCBI GenBank Nucleotide #: NM_022818.4
• UniProt Accession #: Q9GZQ8; Q6NW02
• Molecular Weight: 14,688 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3B
• NCBI Official Synonym Full Names: microtubule associated protein 1 light chain 3 beta
• NCBI Official Symbol: MAP1LC3B
• NCBI Official Synonym Symbols: LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3B
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3B
• UniProt Synonym Gene Names: MAP1ALC3; MAP1A/MAP1B LC3 B

NCBI Description

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Uniprot Description

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.