Typical Testing Data/Standard Curve (for reference only)
Human Adrenergic Receptor Beta 2 (ADRb2) ELISA Kit | ADRb2 elisa kit
Human Adrenergic Receptor Beta 2 (ADRb2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADRb2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADRb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADRb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADRb2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008]
Uniprot Description
ADRB2: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: apical plasma membrane; clathrin-coated vesicle membrane; early endosome; endosome; endosome membrane; integral component of plasma membrane; lysosome; membrane; nucleus; plasma membrane; receptor complex
Molecular Function: adenylate cyclase binding; amyloid-beta binding; beta2-adrenergic receptor activity; epinephrine binding; norepinephrine binding; potassium channel regulator activity; protein binding; protein complex binding; protein homodimerization activity
Biological Process: activation of transmembrane receptor protein tyrosine kinase activity; adenylate cyclase activation; adenylate cyclase-activating adrenergic receptor signaling pathway; adrenergic receptor signaling pathway; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; bone resorption; brown fat cell differentiation; cell surface receptor signaling pathway; cell-cell signaling; cellular response to amyloid-beta; diet induced thermogenesis; endosome to lysosome transport; G-protein coupled receptor signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; heat generation; negative regulation of multicellular organism growth; negative regulation of smooth muscle contraction; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure; positive regulation of AMPA receptor activity; positive regulation of autophagosome maturation; positive regulation of bone mineralization; positive regulation of cAMP-dependent protein kinase activity; positive regulation of lipophagy; positive regulation of MAPK cascade; positive regulation of mini excitatory postsynaptic potential; positive regulation of protein kinase A signaling; positive regulation of protein serine/threonine kinase activity; positive regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; protein deubiquitination; receptor-mediated endocytosis; regulation of sodium ion transport; response to cold; response to psychosocial stress
Disease: Asthma, Susceptibility To; Obesity
Research Articles on ADRb2
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Product Notes
The Human ADRb2 adrb2 (Catalog #AAA456827) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456827 ELISA Kit recognizes Human ADRb2. It is sometimes possible for the material contained within the vial of "Adrenergic Receptor Beta 2 (ADRb2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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