Mouse Progesterone Receptor (PGR) ELISA Kit | PGR elisa kit

Mouse Progesterone Receptor (PGR) ELISA Kit

Cat. Num. AAA456234

• Gene Names: Pgr; PR; PR-A; PR-B; NR3C3; BB114106; 9930019P03Rik; ENSMUSG00000074510
• Reactivity: Mouse
• Synonyms: Progesterone Receptor (PGR); Mouse Progesterone Receptor (PGR) ELISA Kit; NR3C3; PR; Nuclear Receptor Subfamily 3; Group C; Member 3; PGR elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of PGR. No significant cross-reactivity or interference between PGR and analogues was observed.
• Sequence Length: 761

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Competitive inhibition
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.128ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Related Product Information for PGR elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PGR in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGR has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGR and unlabeled PGR (Standards or samples) with the pre-coated antibody specific to PGR. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PGR in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PGR in the sample.

NCBI and Uniprot Product Information

• NCBI GeneID: 18667
• UniProt Accession #: A6H6A5; E9QPW3; Q00175
• Molecular Weight: 81,746 Da
• NCBI Official Synonym Full Names: progesterone receptor
• NCBI Official Symbol: Pgr
• NCBI Official Synonym Symbols: PR; PR-A; PR-B; NR3C3; BB114106; 9930019P03Rik; ENSMUSG00000074510
• NCBI Protein Information: progesterone receptor
• UniProt Protein Name: Progesterone receptor
• Protein Family: Protein
• UniProt Gene Name: Pgr
• UniProt Synonym Gene Names: Nr3c3; Pr; PR

NCBI Description

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. [provided by RefSeq, Sep 2015]

Uniprot Description

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.