Human Calmodulin (CAM) ELISA Kit | CAM elisa kit

Human Calmodulin (CAM) ELISA Kit

Cat. Num. AAA455890

• Gene Names: CALM2; caM; PHKD; CAMII; LQT15; PHKD2
• Reactivity: Human
• Synonyms: Calmodulin (CAM); Human Calmodulin (CAM) ELISA Kit; CALcium MODULated ProteIN; CAM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CAM. No significant cross-reactivity or interference between CAM and analogues was observed.
• Sequence Length: 149

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 93.75-6000pg/mL
• Sensitivity: 34.56pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CAM were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CAM were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAM elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CAM in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 779176942
• NCBI GeneID: 805
• NCBI Accession #: NP_001292553.1
• NCBI GenBank Nucleotide #: NM_001305624.1
• UniProt Accession #: P62158; P02593; P70667; P99014; Q13942; Q53S29; Q61379; Q61380; Q96HK3
• Molecular Weight: 16,838 Da
• NCBI Official Full Name: calmodulin isoform 1
• NCBI Official Synonym Full Names: calmodulin 2
• NCBI Official Symbol: CALM2
• NCBI Official Synonym Symbols: caM; PHKD; CAMII; LQT15; PHKD2
• NCBI Protein Information: calmodulin
• UniProt Protein Name: Calmodulin
• Protein Family: Calmodulin
• UniProt Gene Name: CALM1
• UniProt Synonym Gene Names: CALM; CAM; CAM1; CaM

NCBI Description

This gene is a member of the calmodulin gene family. There are three distinct calmodulin genes dispersed throughout the genome that encode the identical protein, but differ at the nucleotide level. Calmodulin is a calcium binding protein that plays a role in signaling pathways, cell cycle progression and proliferation. Several infants with severe forms of long-QT syndrome (LQTS) who displayed life-threatening ventricular arrhythmias together with delayed neurodevelopment and epilepsy were found to have mutations in either this gene or another member of the calmodulin gene family (PMID:23388215). Mutations in this gene have also been identified in patients with less severe forms of LQTS (PMID:24917665), while mutations in another calmodulin gene family member have been associated with catecholaminergic polymorphic ventricular tachycardia (CPVT)(PMID:23040497), a rare disorder thought to be the cause of a significant fraction of sudden cardiac deaths in young individuals. Pseudogenes of this gene are found on chromosomes 10, 13, and 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.