Human Renalase (RNLS) ELISA Kit | RNLS elisa kit

Human Renalase (RNLS) ELISA Kit

Cat. Num. AAA453648

• Gene Names: RNLS; C10orf59; RENALASE
• Reactivity: Human
• Synonyms: Renalase (RNLS); Human Renalase (RNLS) ELISA Kit; MAO-C; C10orf59; FAD-Dependent Amine Oxidase; Monoamine oxidase-C; alpha-NAD(P)H oxidase/anomerase; RNLS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RNLS. No significant cross-reactivity or interference between RNLS and analogues was observed.
• Sequence Length: 342

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.25-80ng/mL
• Sensitivity: 0.52ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RNLS were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RNLS were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RNLS elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RNLS in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNLS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RNLS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNLS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RNLS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 237649117
• NCBI GeneID: 55328
• NCBI Accession #: NP_001026879.2
• NCBI GenBank Nucleotide #: NM_001031709.2
• UniProt Accession #: Q5VYX0; Q9BS33; Q9NUP8
• Molecular Weight: 34,949 Da
• NCBI Official Full Name: renalase isoform 1
• NCBI Official Synonym Full Names: renalase, FAD dependent amine oxidase
• NCBI Official Symbol: RNLS
• NCBI Official Synonym Symbols: C10orf59; RENALASE
• NCBI Protein Information: renalase
• UniProt Protein Name: Renalase
• UniProt Gene Name: RNLS
• UniProt Synonym Gene Names: C10orf59; MAO-C

NCBI Description

Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney (Xu et al., 2005 [PubMed 15841207]).[supplied by OMIM, Mar 2008]

Uniprot Description

Catalyzes the oxidation of the less abundant 1,2-dihydro-beta-NAD(P) and 1,6-dihydro-beta-NAD(P) to form beta-NAD(P)+. The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.