Human Gamma-Glutamyl Hydrolase (gGH) ELISA Kit | gGH elisa kit

Human Gamma-Glutamyl Hydrolase (gGH) ELISA Kit

Cat. Num. AAA451738

• Gene Names: GGH; GH
• Reactivity: Human
• Synonyms: Gamma-Glutamyl Hydrolase (gGH); Human Gamma-Glutamyl Hydrolase (gGH) ELISA Kit; GH; conjugase; folylpolygammaglutamyl hydrolase; Gamma-Glu-X carboxypeptidase; gGH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GGH. No significant cross-reactivity or interference between GGH and analogues was observed.
• Sequence Length: 318

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GGH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GGH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for gGH elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GGH in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GGH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GGH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GGH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GGH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503987
• NCBI GeneID: 8836
• NCBI Accession #: NP_003869.1
• NCBI GenBank Nucleotide #: NM_003878.2
• UniProt Accession #: Q92820
• Molecular Weight: 35,964 Da
• NCBI Official Full Name: gamma-glutamyl hydrolase
• NCBI Official Synonym Full Names: gamma-glutamyl hydrolase
• NCBI Official Symbol: GGH
• NCBI Official Synonym Symbols: GH
• NCBI Protein Information: gamma-glutamyl hydrolase
• UniProt Protein Name: Gamma-glutamyl hydrolase
• Protein Family: Gamma-glutamyl hydrolase
• UniProt Gene Name: GGH

NCBI Description

This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]

Uniprot Description

Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.