Human Biglycan (BGN) ELISA Kit | BGN elisa kit

Human Biglycan (BGN) ELISA Kit

Cat. Num. AAA451427

• Gene Names: BGN; PGI; MRLS; DSPG1; PG-S1; SEMDX; SLRR1A
• Reactivity: Human
• Synonyms: Biglycan (BGN); Human Biglycan (BGN) ELISA Kit; DSPG1; PG-S1; PGI; SLRR1A; Biglycan Proteoglycan; Bone/cartilage proteoglycan I; BGN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BGN. No significant cross-reactivity or interference between BGN and analogues was observed.
• Sequence Length: 368

• Samples: Serum, plasma or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BGN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BGN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BGN elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of BGN in human serum, plasma or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BGN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BGN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502403
• NCBI GeneID: 633
• NCBI Accession #: NP_001702.1
• NCBI GenBank Nucleotide #: NM_001711.5
• UniProt Accession #: P21810; P13247; D3DWU3
• Molecular Weight: 41,654 Da
• NCBI Official Full Name: biglycan preproprotein
• NCBI Official Synonym Full Names: biglycan
• NCBI Official Symbol: BGN
• NCBI Official Synonym Symbols: PGI; MRLS; DSPG1; PG-S1; SEMDX; SLRR1A
• NCBI Protein Information: biglycan
• UniProt Protein Name: Biglycan
• Protein Family: Biglycan
• UniProt Gene Name: BGN
• UniProt Synonym Gene Names: SLRR1A

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in bone growth, muscle development and regeneration, and collagen fibril assembly in multiple tissues. This protein may also regulate inflammation and innate immunity. Additionally, the encoded protein may contribute to atherosclerosis and aortic valve stenosis in human patients. This gene and the related gene decorin are thought to be the result of a gene duplication. [provided by RefSeq, Nov 2015]

Uniprot Description

May be involved in collagen fiber assembly.