Human Lymphocyte Cytosolic Protein 1 (LCP1) ELISA Kit | LCP1 elisa kit

Human Lymphocyte Cytosolic Protein 1 (LCP1) ELISA Kit

Cat. Num. AAA451418

• Gene Names: LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
• Reactivity: Human
• Synonyms: Lymphocyte Cytosolic Protein 1 (LCP1); Human Lymphocyte Cytosolic Protein 1 (LCP1) ELISA Kit; CP64; LC64P; PLS2; L-Plastin; Plastin 2; LCP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LCP1. No significant cross-reactivity or interference between LCP1 and analogues was observed.
• Sequence Length: 627

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LCP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LCP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCP1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LCP1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LCP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 167614506
• NCBI GeneID: 3936
• NCBI Accession #: NP_002289.2
• NCBI GenBank Nucleotide #: NM_002298.4
• UniProt Accession #: P13796; Q5TBN4; B2R613; B4DUA0
• Molecular Weight: 21,792 Da
• NCBI Official Full Name: plastin-2
• NCBI Official Synonym Full Names: lymphocyte cytosolic protein 1
• NCBI Official Symbol: LCP1
• NCBI Official Synonym Symbols: LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
• NCBI Protein Information: plastin-2
• UniProt Protein Name: Plastin-2
• Protein Family: Larval cuticle protein
• UniProt Gene Name: LCP1
• UniProt Synonym Gene Names: PLS2; LCP-1

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.