Human Beclin 1 (BECN1) ELISA Kit | BECN1 elisa kit

Human Beclin 1 (BECN1) ELISA Kit

Cat. Num. AAA451360

• Gene Names: BECN1; ATG6; VPS30; beclin1
• Reactivity: Human
• Synonyms: Beclin 1 (BECN1); Human Beclin 1 (BECN1) ELISA Kit; ATG6; VPS30; Coiled-Coil; Myosin-Like BCL2 Interacting Protein; ATG6 Autophagy Related 6 Homolog; BECN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BECN1. No significant cross-reactivity or interference between BECN1 and analogues was observed.
• Sequence Length: 450

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BECN1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BECN1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BECN1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BECN1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BECN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BECN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BECN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BECN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 929524267
• NCBI GeneID: 8678
• NCBI Accession #: NP_001300927.1
• NCBI GenBank Nucleotide #: NM_001313998.1
• UniProt Accession #: Q14457; O75595; Q9UNA8; B2R6N7
• Molecular Weight: 51,896 Da
• NCBI Official Full Name: beclin-1 isoform a
• NCBI Official Synonym Full Names: beclin 1
• NCBI Official Symbol: BECN1
• NCBI Official Synonym Symbols: ATG6; VPS30; beclin1
• NCBI Protein Information: beclin-1
• UniProt Protein Name: Beclin-1
• Protein Family: Beclin
• UniProt Gene Name: BECN1
• UniProt Synonym Gene Names: GT197

NCBI Description

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.