Human Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit | SGLT1 elisa kit

Human Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit

Cat. Num. AAA451154

• Gene Names: SLC5A1; NAGT; SGLT1; D22S675
• Reactivity: Human
• Synonyms: Sodium/Glucose Cotransporter 1 (SGLT1); Human Sodium/Glucose Cotransporter 1 (SGLT1) ELISA Kit; SLC5A1; NAGT; Solute Carrier Family 5 Member 1; High affinity sodium-glucose cotransporter; Na(+)/glucose cotransporter 1; SGLT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SGLT1. No significant cross-reactivity or interference between SGLT1 and analogues was observed.
• Sequence Length: 664

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SGLT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SGLT1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SGLT1 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SGLT1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SGLT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SGLT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SGLT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SGLT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507031
• NCBI GeneID: 6523
• NCBI Accession #: NP_000334.1
• NCBI GenBank Nucleotide #: NM_000343.3
• UniProt Accession #: P13866; B2R7E2; B7Z4Q9; B7ZA69
• Molecular Weight: 60,097 Da
• NCBI Official Full Name: sodium/glucose cotransporter 1 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 5 member 1
• NCBI Official Symbol: SLC5A1
• NCBI Official Synonym Symbols: NAGT; SGLT1; D22S675
• NCBI Protein Information: sodium/glucose cotransporter 1
• UniProt Protein Name: Sodium/glucose cotransporter 1
• UniProt Gene Name: SLC5A1
• UniProt Synonym Gene Names: NAGT; SGLT1; Na(+)/glucose cotransporter 1

NCBI Description

This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Actively transports glucose into cells by Na+ cotransport with a Na+ to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na+/glucose cotransporter arranged in series along kidney proximal tubules.