Citrate Synthase (CS) RTU ELISA Kit | CS rtu elisa kit

Rat Citrate Synthase (CS) ELISA Kit

Cat. Num. AAA4503895

• Reactivity: Rat
• Synonyms: Citrate Synthase (CS); Rat Citrate Synthase (CS) ELISA Kit; Citrate (Si)-synthase; mitochondrial; CS rtu elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CRISPLD2. No significant cross-reactivity or interference between CRISPLD2 and analogues was observed.
• Sequence Length: 466

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12-200ng/mL
• Sensitivity: 1.12ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CRISPLD2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CRISPLD2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CS rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CRISPLD2 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRISPLD2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CRISPLD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRISPLD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CRISPLD2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18543177
• NCBI GeneID: 170587
• NCBI Accession #: NP_570111.1
• NCBI GenBank Nucleotide #: NP_570111.1
• UniProt Accession #: Q8VHF5
• NCBI Official Full Name: citrate synthase, mitochondrial
• NCBI Official Synonym Full Names: citrate synthase
• NCBI Official Symbol: Cs
• NCBI Protein Information: citrate synthase, mitochondrial
• UniProt Protein Name: Citrate synthase, mitochondrial
• UniProt Gene Name: Cs

NCBI Description

human homolog is an enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A [RGD, Feb 2006]

Uniprot Description

CS: a mitochondrial enzyme of the tricarboxylic acid cycle that converts acetyl-CoA + H2O + oxaloacetate into citrate + CoA. Found in nearly all cells capable of oxidative metabolism.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 2.3.3.1; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 7q11

Cellular Component: mitochondrial matrix; mitochondrion; nucleus

Molecular Function: citrate (Si)-synthase activity; RNA binding; transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer

Biological Process: acetyl-CoA metabolic process; carbohydrate metabolic process; citrate metabolic process; oxaloacetate metabolic process; tricarboxylic acid cycle