Human Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit | PDIA6 elisa kit

Human Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit

Cat. Num. AAA450296

• Gene Names: PDIA6; P5; ERP5; TXNDC7
• Reactivity: Human
• Synonyms: Protein Disulfide Isomerase A6 (PDIA6); Human Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit; PDI-A6; TXNDC7; P5; ERp5; Thioredoxin Domain Containing 7(Protein Disulfide Isomerase); Protein Disulfide Isomerase-Associated 6; Endoplasmic reticulum protein 5; PDIA6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PDIA6. No significant cross-reactivity or interference between PDIA6 and analogues was observed.
• Sequence Length: 492

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.049ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PDIA6 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PDIA6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDIA6 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDIA6 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDIA6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDIA6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDIA6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PDIA6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 544186032
• NCBI GeneID: 10130
• NCBI Accession #: NP_001269633.1
• NCBI GenBank Nucleotide #: NM_001282704.1
• UniProt Accession #: Q15084; Q53RC7; Q6ZSH5; Q99778; B3KY95; B5MCQ5; B7Z254; B7Z4M8; F8WA83
• Molecular Weight: 53,261 Da
• NCBI Official Full Name: protein disulfide-isomerase A6 isoform a
• NCBI Official Synonym Full Names: protein disulfide isomerase family A member 6
• NCBI Official Symbol: PDIA6
• NCBI Official Synonym Symbols: P5; ERP5; TXNDC7
• NCBI Protein Information: protein disulfide-isomerase A6
• UniProt Protein Name: Protein disulfide-isomerase A6
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA6
• UniProt Synonym Gene Names: ERP5; P5; TXNDC7; ER protein 5; ERp5

NCBI Description

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]

Uniprot Description

May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.