Human Alpha-Fodrin (SPTAN1) RTU ELISA Kit | SPTAN1 rtu elisa kit

Human Alpha-Fodrin (SPTAN1) ELISA Kit

Cat. Num. AAA4502634

• Gene Names: SPTAN1; NEAS; EIEE5; SPTA2
• Reactivity: Human
• Synonyms: Alpha-Fodrin (SPTAN1); Human Alpha-Fodrin (SPTAN1) ELISA Kit; SPTAN1; NEAS; (ALPHA)II-SPECTRIN; Spectrin Alpha Non-Erythrocytic 1; Spectrin Alpha Chain; Brain; Spectrin; non-erythroid alpha subunit; SPTAN1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 2477

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPTAN1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 194595509
• NCBI GeneID: 6709
• NCBI Accession #: NP_001123910.1
• NCBI GenBank Nucleotide #: NM_001130438.2
• UniProt Accession #: Q13813; Q13186; Q15324; Q16606; Q59EF1; Q5VXV5; Q5VXV6; Q7Z6M5; Q9P0V0
• Molecular Weight: 282,282 Da
• NCBI Official Full Name: spectrin alpha chain, non-erythrocytic 1 isoform 1
• NCBI Official Synonym Full Names: spectrin alpha, non-erythrocytic 1
• NCBI Official Symbol: SPTAN1
• NCBI Official Synonym Symbols: NEAS; EIEE5; SPTA2
• NCBI Protein Information: spectrin alpha chain, non-erythrocytic 1
• UniProt Protein Name: Spectrin alpha chain, non-erythrocytic 1
• Protein Family: Spectrin
• UniProt Gene Name: SPTAN1
• UniProt Synonym Gene Names: NEAS; SPTA2

NCBI Description

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.