Mouse Matrix Metalloproteinase 9 (MMP9) RTU ELISA Kit | MMP9 rtu elisa kit

Mouse Matrix Metalloproteinase 9 (MMP9) ELISA Kit

Cat. Num. AAA4501117

• Gene Names: Mmp9; Clg4b; Gel B; MMP-9; B/MMP9; AW743869; pro-MMP-9
• Reactivity: Mouse
• Synonyms: Matrix Metalloproteinase 9 (MMP9); Mouse Matrix Metalloproteinase 9 (MMP9) ELISA Kit; GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase; MMP9 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 730

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.069ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP9 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MMP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MMP9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7305277
• NCBI GeneID: 17395
• NCBI Accession #: NP_038627.1
• NCBI GenBank Nucleotide #: NM_013599.4
• UniProt Accession #: P41245; Q06788; Q80XI8; Q9DC02
• Molecular Weight: 80,535 Da
• NCBI Official Full Name: matrix metalloproteinase-9 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 9
• NCBI Official Symbol: Mmp9
• NCBI Official Synonym Symbols: Clg4b; Gel B; MMP-9; B/MMP9; AW743869; pro-MMP-9
• NCBI Protein Information: matrix metalloproteinase-9
• UniProt Protein Name: Matrix metalloproteinase-9
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp9
• UniProt Synonym Gene Names: Clg4b; MMP-9; GELB

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mice lacking the encoded protein exhibit an abnormal pattern of skeletal growth plate vascularization and ossification, reduced keratinocyte hyperproliferation at all neoplastic stages, a decreased incidence of invasive tumors, and resistance to experimental autoimmune encephalomyelitis. [provided by RefSeq, Feb 2016]

Uniprot Description

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments ().