Rabbit AMACR/p504S Monoclonal Antibody | anti-AMACR antibody

AMACR/p504S (Prostate Cancer Marker)

Cat. Num. AAA439974

• Gene Names: AMACR; RM; RACE; CBAS4; P504S; AMACRD
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry
• Synonyms: AMACR/p504S; Monoclonal Antibody; AMACR/p504S (Prostate Cancer Marker); Alpha-methylacyl-CoA Racemase; CBAS4; Da1-8; Macr1; RACE; RM; anti-AMACR antibody

• Host: Rabbit
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG, kappa
• Clone Number: 13H4
• Specificity: This antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
• Form/Format: Culture Supernatant with 0.05% Azide.
• Sequence Length: 394

• Applicable Applications for anti-AMACR antibody: Immunohistochemistry (IHC) Formalin
• Application Notes: IHC-F: 1:50-1:100 for 30-60 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Full length human recombinant AMACR protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 5p13.3
• Positive Control: HEK cells or Prostate Adenocarcinoma
• Preparation and Storage: Antibody with azide - store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR / p504S Rabbit Monoclonal Antibody (13H4))

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR / p504S Rabbit Monoclonal Antibody (13H4))

References

Xu J et. al. Canc Res. 2000; 60:1677. Jiang Z et. al. Human Pathology. 2003; 34(8):792. Jiang Z et. al. Am J Surg Pathol. 2001; 25(11):139.

NCBI and Uniprot Product Information

• NCBI GI #: 266456254
• NCBI GeneID: 23600
• NCBI Accession #: NP_001161067.1
• NCBI GenBank Nucleotide #: NM_001167595.1
• UniProt Accession #: Q9UHK6; O43673; Q3KT79; Q96GH1; Q9Y3Q1; A5YM47; B8Y916; B8Y918; F8W9N1
• Molecular Weight: 54kDa
• NCBI Official Full Name: alpha-methylacyl-CoA racemase isoform 3
• NCBI Official Synonym Full Names: alpha-methylacyl-CoA racemase
• NCBI Official Symbol: AMACR
• NCBI Official Synonym Symbols: RM; RACE; CBAS4; P504S; AMACRD
• NCBI Protein Information: alpha-methylacyl-CoA racemase
• UniProt Protein Name: Alpha-methylacyl-CoA racemase
• Protein Family: Alpha-methylacyl-CoA racemase
• UniProt Gene Name: AMACR

NCBI Description

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.