Rabbit anti-Human CYP11A1 Polyclonal Antibody | anti-CYP11A1 antibody

CYP11A1, NT (Cholesterol Side-chain Cleavage Enzyme, Mitochondrial, CYPXIA1, Cytochrome P450 11A1, CYPXIA1, Cytochrome P450(scc), Cholesterol Desmolase, CYP11A) (AP)

Cat. Num. AAA6471635

• Gene Names: CYP11A1; CYP11A; CYPXIA1; P450SCC
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP11A1; Polyclonal Antibody; NT (Cholesterol Side-chain Cleavage Enzyme; Mitochondrial; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450(scc); Cholesterol Desmolase; CYP11A) (AP); anti-CYP11A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP11A1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CYP11A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CYP11A1 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CYP11A1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). CYP11A1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CYP11A1 antibody

Antibodies; Enzymes; Cytochrome

References

1. Celhar, T., Cancer Genet. Cytogenet. 187(1), 28-33 (2008). 2. Morales, A., Pancreas 37(2), 165-169 (2008). 3. Shigematsu, K., Eur. J. Endocrinol. 158(6), 867-878 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 153218646
• NCBI GeneID: 1583
• NCBI Accession #: NP_000772
• NCBI GenBank Nucleotide #: NM_000781
• UniProt Accession #: P05108
• Molecular Weight: 57kDa
• NCBI Official Full Name: cholesterol side-chain cleavage enzyme, mitochondrial isoform a
• NCBI Official Synonym Full Names: cytochrome P450 family 11 subfamily A member 1
• NCBI Official Symbol: CYP11A1
• NCBI Official Synonym Symbols: CYP11A; CYPXIA1; P450SCC
• NCBI Protein Information: cholesterol side-chain cleavage enzyme, mitochondrial
• UniProt Protein Name: Cholesterol side-chain cleavage enzyme, mitochondrial
• Protein Family: Cholesterol side-chain cleavage enzyme
• UniProt Gene Name: CYP11A1
• UniProt Synonym Gene Names: CYP11A
• UniProt Entry Name: CP11A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP11A1: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR). A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.15.6; Mitochondrial; Lipid Metabolism - C21-steroid hormone; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: mitochondrial crista; mitochondrion; mitochondrial matrix; perikaryon

Molecular Function: cholesterol monooxygenase (side-chain-cleaving) activity; iron ion binding; cholesterol binding; heme binding

Biological Process: response to alkaloid; steroid metabolic process; maternal process involved in pregnancy; dibenzo-p-dioxin metabolic process; response to insecticide; response to L-ascorbic acid; estrogen biosynthetic process; response to salt stress; response to vitamin E; Leydig cell differentiation; biphenyl metabolic process; response to gamma radiation; cerebellum development; response to corticosterone stimulus; response to drug; cholesterol metabolic process; progesterone biosynthetic process; fractalkine metabolic process; hippocampus development; phthalate metabolic process; mating behavior; vitamin D metabolic process; Schwann cell differentiation; response to genistein; response to hydrogen peroxide; xenobiotic metabolic process; C21-steroid hormone biosynthetic process; sterol metabolic process; granulosa cell differentiation; phenol metabolic process

Disease: Adrenal Insufficiency, Congenital, With 46,xy Sex Reversal, Partial Or Complete