Mouse Cytochrome p450 Monoclonal Antibody | anti-CYP2E1 antibody

Cytochrome p450

Cat. Num. AAA439906

• Gene Names: CYP2E1; CPE1; CYP2E; P450-J; P450C2E
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry
• Synonyms: Cytochrome p450; Monoclonal Antibody; 4-nitrophenol 2-hydroxylase; CPE1; CYP2Ecytochrome P450 2E1; CYPIIE1; cytochrome P450; family 2; subfamily E; polypeptide 1; subfamily IIE (ethanol-inducible); Cytochrome P450-J; EC 1.14.13; EC 1.14.13.n7; EC 1.14.14.1; flavoprotein-linked monooxygenase; microsomal monooxygenase; P450C2E; P450-J; xenobiotic monooxygenase; anti-CYP2E1 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M12P4H2
• Specificity: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are mono-oxygenases, which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines, which are pre-mutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.
• Sequence Length: 493

• Applicable Applications for anti-CYP2E1 antibody: Immunohistochemistry (IHC) Formalin
• Application Notes: IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Ovalbumin-conjugated synthetic peptide HIGFGCIPPR (C-terminal sequence)
• Cellular Localization: Cytoplasmic (Endoplasmic Reticulum)
• Hu-chromosome Location: 10q26.3
• Positive Control: HepG2 Cells. Hepatic Carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Hepatic Carcinoma stained with Cytochrome p450 Mouse Monoclonal Antibody (M12P4H2).)

SDS-Page

(SDS-PAGE Analysis Purified Cytochrome p450 Mouse Monoclonal Antibody (M12P4H2). Confirmation of Integrity and Purity of Antibody.)

References

Itoga, S., Nomura, F., Harada, S., Tsutsumi, M., Takase, S. and Nakai, T. 1999. Mutations in the exons and exon-intron junction regions of human cytochrome P-4502E1 gene and alcoholism. Alcohol. Clin. Exp. Res. 23: 13S-16S.

NCBI and Uniprot Product Information

• NCBI GI #: 10834998
• NCBI GeneID: 1571
• NCBI Accession #: NP_000764.1
• NCBI GenBank Nucleotide #: NM_000773.3
• UniProt Accession #: P05181; Q5VZD5; Q6NWT9; Q9UK47
• Molecular Weight: 50-55kDa
• NCBI Official Full Name: cytochrome P450 2E1
• NCBI Official Synonym Full Names: cytochrome P450 family 2 subfamily E member 1
• NCBI Official Symbol: CYP2E1
• NCBI Official Synonym Symbols: CPE1; CYP2E; P450-J; P450C2E
• NCBI Protein Information: cytochrome P450 2E1
• UniProt Protein Name: Cytochrome P450 2E1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2E1
• UniProt Synonym Gene Names: CYP2E

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.