Mouse anti-Human CD38 Monoclonal Antibody | anti-CD38 antibody

CD38 (ADP Ribosyl Cyclase I) Mouse Monoclonal Antibody

Cat. Num. AAA439039

• Gene Names: CD38; ADPRC1; ADPRC 1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD38; Monoclonal Antibody; CD38 (ADP Ribosyl Cyclase I) Mouse Monoclonal Antibody; Acute Lymphoblastic Leukemia Cells Antigen; ADP Ribosyl Cyclase 1; cADP-ribose Hydrolase 1; CD38H; NAD(+) Nucleosidase; NIM-R5 Antigen; p45; T10; anti-CD38 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG3, kappa
• Clone Number: AT2
• Specificity: This MAb reacts with a 45kDa glycopeptide, which is a type II membrane glycoprotein with a transmembrane sequence near the NH2terminus. CD38 is a type II transmembrane glycoprotein that is present on early B- and T-cell lineages and activated B- and T-cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B-cells, mitogen-activated T-cells, monocytes and Ig-secreting plasma cells. CD38 is expressed on CD34+ cells. The CD34+CD38- population of hematopoietic stems cells defines the most pluripotent cells (e.g. blast colony forming cells).
• Form/Format: 0.5 ml at 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide.
• Sequence Length: 300

• Applicable Applications for anti-CD38 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
• Cellular Localization: Cell Surface, Cytoplasm and nucleus
• Immunogen: Human T cell line CCRF-CEM
• Hu-Chromosome Location: 4p15
• Positive Control: CCRF-CEM cells, Tonsil, Spleen or Skin
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months.

References

Deaglio S et. al. J Immunol. 1998;160(1):395-402

NCBI and Uniprot Product Information

• NCBI GI #: 38454326
• NCBI GeneID: 952
• NCBI Accession #: NP_001766.2
• NCBI GenBank Nucleotide #: NM_001775.3
• UniProt Accession #: P28907; O00121; O00122; Q96HY4
• Molecular Weight: ~45kDa (Glycoprotein); 35kDa (protein core)
• NCBI Official Full Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: ADPRC1; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• Protein Family: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1
• UniProt Entry Name: CD38_HUMAN

NCBI Description

The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Lyase; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.4.99.20; Membrane protein, integral; Cell surface; Hydrolase; Apoptosis; EC 3.2.2.6

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: membrane

Molecular Function: NAD+ nucleosidase activity; phosphorus-oxygen lyase activity

Biological Process: B cell receptor signaling pathway; negative regulation of apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of transcription, DNA-dependent; response to drug