Mouse Nucleolin Monoclonal Antibody | anti-NCL antibody

Nucleolin (Marker of Human Cells) Mouse Monoclonal Antibody

Cat. Num. AAA438961

• Gene Names: NCL; C23
• Reactivity: Human.; Does not react with Mouse, Rat and Cow
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: Nucleolin; Monoclonal Antibody; Nucleolin (Marker of Human Cells) Mouse Monoclonal Antibody; NCL; Nucl; Protein C23; anti-NCL antibody

• Host: Mouse
• Reactivity: Human.; Does not react with Mouse, Rat and Cow
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM614
• Specificity: Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 710

• Applicable Applications for anti-NCL antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Nucleoli
• Immunogen: Recombinant human NCL protein
• Hu-Chromosome Location: 2q37.1
• Positive Control: All human Cells. Testis, Ovary, Uterus, Endometrial or Hodgkin's lymphoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).)

References

Fujiki H, Watanabe T, Suganuma M. Cell-surface nucleolin acts as a central mediator for carcinogenic, anti-carcinogenic, and disease-related ligands. J Cancer Res Clin Oncol. 2014;140(5):689-99. Qiu W, Zhou F, Zhang Q, Sun X, Shi X, Liang Y, Wang X, Yue L. Overexpression of nucleolin and different expression sites both related to the prognosis of gastric cancer. APMIS. 2013;121(10):919-25.

NCBI and Uniprot Product Information

• NCBI GI #: 55956788
• NCBI GeneID: 4691
• NCBI Accession #: NP_005372.2
• NCBI GenBank Nucleotide #: NM_005381.2
• UniProt Accession #: P19338; Q53SK1; Q8NB06; Q9UCF0; Q9UDG1
• Molecular Weight: 76kDa
• NCBI Official Full Name: nucleolin
• NCBI Official Synonym Full Names: nucleolin
• NCBI Official Symbol: NCL
• NCBI Official Synonym Symbols: C23
• NCBI Protein Information: nucleolin
• UniProt Protein Name: Nucleolin
• Protein Family: Nucleolin
• UniProt Gene Name: NCL
• UniProt Entry Name: NUCL_HUMAN

NCBI Description

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Uniprot Description

NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.

Protein type: Helicase; Nucleolus; RNA-binding; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cell cortex; membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: identical protein binding; protein binding; protein C-terminus binding; RNA binding; telomeric DNA binding

Biological Process: angiogenesis