Mouse anti-Human. CD171 Monoclonal Antibody | anti-L1CAM antibody

CD171/ NCAM-L1

Cat. Num. AAA438259

• Gene Names: L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Reactivity: Human.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry
• Synonyms: CD171; Monoclonal Antibody; CD171/ NCAM-L1; CAML1; HSAS1; Hyd; L1 Cell Adhesion Molecule; L1-NCAM; MASA; MIC5; NCAM-L1; Nerve-growth factor-inducible large external glycoprotein; Neural cell adhesion molecule L1; NILE; S10; SPG1; anti-L1CAM antibody

• Host: Mouse
• Reactivity: Human.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: UJ127
• Specificity: Recognizes a cell surface protein of 220-240kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This MAb is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain.
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
• Sequence Length: 1257

• Applicable Applications for anti-L1CAM antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB), Immunohistology (IH)
• Application Notes: FC: (1-2ug/million cells); IF: (1-2ug/ml); Optimal dilution for a specific application should be determined.
• Localization: Cell surface
• Positive Control: Neuroblastomas or Schwannomas
• Human Chromosomal Location: Xq28
• Immunogen: Homogenous suspension of 16 week human fetal brain
• Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide
• Preparation and Storage: Antibody with azide store at 2 to 8°C. Antibody without azide store at -20 to -80°C

SDS-PAGE

(SDS-PAGE Analysis Purified CD171 Mouse Monoclonal Antibody (UJ127). Confirmation of Integrity and Purity of Antibody.)

References

1. Kemshead J, et. al. International J Cancer, 1983; 31:187-195.
2. Patel, et. al. Biochem Soc Transactions, 1990; 18:274

NCBI and Uniprot Product Information

• NCBI GI #: 4557707
• NCBI GeneID: 3897
• NCBI Accession #: NP_000416.1
• NCBI GenBank Nucleotide #: NM_000425.4
• UniProt Accession #: P32004; Q8TA87; A0AV65; A4ZYW4; B2RMU7; G3XAF4
• Molecular Weight: 220-240kDa
• NCBI Official Full Name: neural cell adhesion molecule L1 isoform 1
• NCBI Official Synonym Full Names: L1 cell adhesion molecule
• NCBI Official Symbol: L1CAM
• NCBI Official Synonym Symbols: S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• NCBI Protein Information: neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1
• UniProt Protein Name: Neural cell adhesion molecule L1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: L1CAM
• UniProt Synonym Gene Names: CAML1; MIC5; N-CAM-L1; NCAM-L1
• UniProt Entry Name: L1CAM_HUMAN

NCBI Description

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Uniprot Description

NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: presynaptic membrane; cell surface; focal adhesion; plasma membrane; integral to membrane; terminal button; external side of plasma membrane

Molecular Function: integrin binding; identical protein binding; protein self-association; sialic acid binding

Biological Process: nervous system development; axon guidance; positive regulation of calcium-mediated signaling; chemotaxis; heterophilic cell adhesion; leukocyte adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; cell-cell adhesion mediated by integrin; positive regulation of cell-cell adhesion; blood coagulation; cell adhesion; homophilic cell adhesion; leukocyte migration

Disease: Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Corpus Callosum, Partial Agenesis Of, X-linked; Masa Syndrome