Mouse anti-Human Ubiquitin-conjugating Enzyme E2C/UBCH10 Monoclonal Antibody | anti-UBE2C antibody

Ubiquitin-conjugating Enzyme E2C/UBCH10

Cat. Num. AAA4381571

• Gene Names: UBE2C; UBCH10; dJ447F3.2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Ubiquitin-conjugating Enzyme E2C/UBCH10; Monoclonal Antibody; Cyclin selective ubiquitin carrier protein; mitotic specific ubiquitin conjugating enzyme; UB E2C; UBCH10; Ubiquitin protein ligase C; anti-UBE2C antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: CPTC-UBE2C-1
• Specificity: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ubconjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 132

• Applicable Applications for anti-UBE2C antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: For coating, order antibody without BSA; IHC-P: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes
• Immunogen: Recombinant Human full-length UBE2C protein
• Cellular Localization: Cytoplasm
• Hu-chromosome Location: 20q13.12
• Positive Control: HeLa, Jurkat or A431 cells. Human Tonsil or Placenta tissue.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)-Formallin

(Formalin-fixed, paraffin-embedded human brain stained with UBE2C Mouse Monoclonal Antibody (CPTC-UBE2C-1))

SDS-Page

(SDS-PAGE Analysis Purified UBE2C Mouse Monoclonal Antibody (CPTC-UBE2C-1). Confirmation of Integrity and Purity of Antibody.)

References

Lin, Y., et al. 2002. Structural and functional analysis of the human mitoticspecific ubiquitin-conjugating enzyme, UBCH10. J. Biol. Chem. 277: 21913-21921.

NCBI and Uniprot Product Information

• NCBI GI #: 528524529
• NCBI GeneID: 11065
• NCBI Accession #: NP_001268670.1
• NCBI GenBank Nucleotide #: NP_001268670.1
• UniProt Accession #: O00762
• Molecular Weight: 20kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 C isoform 6
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 C
• NCBI Official Symbol: UBE2C
• NCBI Official Synonym Symbols: UBCH10; dJ447F3.2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 C
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 C
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2C
• UniProt Synonym Gene Names: UBCH10
• UniProt Entry Name: UBE2C_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]

Uniprot Description

UBE2C: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; Ligase; Cell cycle regulation; EC 6.3.2.19; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; anaphase-promoting complex; cytoplasm; cytosol

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; protein ubiquitination; cyclin catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; exit from mitosis; positive regulation of exit from mitosis