Rat CD40 Monoclonal Antibody | anti-CD40 antibody

CD40, Mouse

Cat. Num. AAA4381532

• Gene Names: Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
• Reactivity: Mouse; Does not react with Human.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD40; Monoclonal Antibody; Mouse; B cell surface antigen CD40; CD40 type II isoform; p50; Bp50; CD40L receptor; CDW40; TRAP; IMD3; IGM; Tnfrsf5; HIGM1; GP39; T-BAM; anti-CD40 antibody

• Host: Rat
• Reactivity: Mouse; Does not react with Human.
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: FGK45.5
• Specificity: Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs. CD40 plays roles in B cell activation, differentiation, proliferation and Ig isotype switching as well as dendritic cell maturation. FGK4.5 can be used for blocking of CD40/CD154 interaction and forin vitroandin vivoactivation of CD40 expressing APCs. This MAb is shown to indirectly activate natural killer (NK) cells, producing significant antitumor and anti-metastatic effects. Effective in boosting immune responses against infectious agents and can potentially be used to treat chronic autoimmune inflammatory processes.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 289

• Applicable Applications for anti-CD40 antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Functional Studies: Order Antibody without BSA & Azide; FC/FACS: 0.5-1ug/million cells in 0.1ml; IF: 0.5-1ug/ml
• Positive Control: MPBMC's. Spleen, Thymus or Lymph Node.
• Immunogen: Purified recombinant Mouse CD40 fusion protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 2 H3; 2 85.38 cM (Mouse)
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Agonistic Anti-CD40 Antibody Profoundly Suppresses the Immune Response to Infection with Lymphocytic Choriomeningitis Virus C. Bartholdy, et al. J. Immunol. 178 1662 (2007)

NCBI and Uniprot Product Information

• NCBI GI #: 172046819
• NCBI GeneID: 21939
• UniProt Accession #: P27512
• Molecular Weight: 48kDa
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 5
• NCBI Official Synonym Full Names: CD40 antigen
• NCBI Official Symbol: Cd40
• NCBI Official Synonym Symbols: IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• Protein Family: CD40 ligand
• UniProt Gene Name: Cd40
• UniProt Synonym Gene Names: Tnfrsf5
• UniProt Entry Name: TNR5_MOUSE

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: extracellular space; cell surface; membrane; intracellular membrane-bound organelle; cell; cytoplasm; integral to membrane; plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; antigen binding

Biological Process: positive regulation of isotype switching to IgG isotypes; regulation of immune response; B cell activation; immune system process; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; protein kinase B signaling cascade; positive regulation of B cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; inflammatory response; defense response to virus; regulation of immunoglobulin secretion