Mouse Cyclin A1 Monoclonal Antibody | anti-CCNA1 antibody

Cyclin A1

Cat. Num. AAA4381478

• Gene Names: ccna1.S; ccna1; Cyclin-A1
• Reactivity: Xenopus laevis
• Applications: Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cyclin A1; Monoclonal Antibody; CCN1; CCNA; CCNA2; anti-CCNA1 antibody

• Host: Mouse
• Reactivity: Xenopus laevis
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: XLA1-3
• Specificity: The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. The antibody detects Xenopus cyclin A in blots. Cyclin A1 is the embryonic cyclin which is present in Xenopus eggs. This MAb only detects Xenopus Cyclin A1.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 418

• Applicable Applications for anti-CCNA1 antibody: Western Blot (WB)
• Application Notes: WB: 1-2ug/ml
• Positive Control: Very high levels in testis. Also found in myeloid leukemia cell lines. Xenopus laevis tissue lysate.
• Immunogen: Recombinant full-length Xenopus laevis Cyclin A1 protein
• Cellular Localization: Nucleus.
• Hu-chromosome Location: Chr02 (Xenopus laevis)
• Preparation and Storage: Antibody with azide: store at 2 to 8°C.; Antibody without azide: store at -20 to -80°C.; Antibody is stable for up to 24 months. Non-hazardous. No MSDS required.

Western Blot (WB)

(Western Blot Analysis of (1) HeLa (2) Jurkat and (3) MCF-7 cell lysates using Cyclin A1 Mouse Monoclonal Antibody (XLA1-3).)

SDS-Page

(SDS-PAGE Analysis Purified Cyclin A1 Mouse Monoclonal Antibody (XLA1-3). Confirmation of Purity and Integrity of Antibody.)

References

Parida, P.K., et al. 2018. Inhibition of cancer progression by a novel transstilbene derivative through disruption of microtubule dynamics, driving G2/M arrest, and p53-dependent apoptosis. Cell Death Dis. 9: 448.

NCBI and Uniprot Product Information

• NCBI GI #: 116172
• NCBI GeneID: 397885
• UniProt Accession #: P18606
• Molecular Weight: 65kDa
• NCBI Official Full Name: Cyclin-A1
• NCBI Official Synonym Full Names: cyclin A1 S homeolog
• NCBI Official Symbol: ccna1.S
• NCBI Official Synonym Symbols: ccna1; Cyclin-A1
• NCBI Protein Information: cyclin-A1
• UniProt Protein Name: Cyclin-A1
• Protein Family: Cyclin
• UniProt Gene Name: ccna1

Uniprot Description

May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions.