Mouse Ku Monoclonal Antibody | anti-Ku antibody

Ku (p70/p80) (Nuclear Marker)

Cat. Num. AAA4380928

• Gene Names: XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• Reactivity: Human, Primates; Does not react with Mouse, Rat or Chicken.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab conjugated to CF488
• Synonyms: Ku; Monoclonal Antibody; Ku (p70/p80) (Nuclear Marker); Ku (p70): 70kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70kDa subunit; CTC box-binding factor 75kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku autoantigen; 70kDa; Ku70; Kup70; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen); Thyroid-lupus autoantigen (TLAA); X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80): 86kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 86Kd subunit; ATP-dependent DNA helicase II 80kDa subunit; CTC box-binding factor 85kDa subunit; CTC85; DNA repair protein XRCC5; KARP1; Ku autoantigen 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; Lupus Ku autoantigen protein p86; Nuclear factor IV (NFIV); X-ray repair cross-complementing protein 5 (XRCC5); anti-Ku antibody

• Host: Mouse
• Reactivity: Human, Primates; Does not react with Mouse, Rat or Chicken.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: KU729
• Specificity: Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. MAb KU729 recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
• Sequence Length: 609

• Applicable Applications for anti-Ku antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 5ul per test per one million cells or 5ul per 100ul of whole blood; IF: 1:50-1:100
• Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells.
• Immunogen: Nuclear extract of human HL-60 cells
• Cellular Localization: Nuclear
• Hu-chromosome Location: 22q13.2 (p70); 2q35 (p80)
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometry of Human Ku (p70/p80) on 293T cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled Ku Monoclonal Antibody (KU729).)

NCBI and Uniprot Product Information

• NCBI GI #: 4503841
• NCBI GeneID: 2547
• NCBI Accession #: NP_001460.1
• NCBI GenBank Nucleotide #: NP_001460.1
• UniProt Accession #: P12956; P13010
• Molecular Weight: 70kDa & 80kDa
• NCBI Official Full Name: X-ray repair cross-complementing protein 6 isoform 1
• NCBI Official Synonym Full Names: X-ray repair cross complementing 6
• NCBI Official Symbol: XRCC6
• NCBI Official Synonym Symbols: ML8; KU70; TLAA; CTC75; CTCBF; G22P1
• NCBI Protein Information: X-ray repair cross-complementing protein 6
• UniProt Protein Name: X-ray repair cross-complementing protein 6
• Protein Family: Kurtoxin
• UniProt Gene Name: XRCC6
• UniProt Synonym Gene Names: G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA
• UniProt Entry Name: XRCC6_HUMAN

NCBI Description

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: Helicase; EC 3.6.4.-; DNA-binding; DNA repair, damage; Nuclear receptor co-regulator; EC 4.2.99.-; RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; transcription factor complex; nuclear telomere cap complex; membrane; nucleolus; cytosol; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; double-stranded DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: positive regulation of neurogenesis; V(D)J recombination; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA repair; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA ligation; double-strand break repair; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance