Mouse anti-Human CD21 Antibody | anti-CD21 antibody

CD21 (Mature B-Cell & Follicular Dendritic Cell Marker)

Cat. Num. AAA4380812

• Gene Names: CR2; CR; C3DR; CD21; CVID7; SLEB9
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD21; CD21 (Mature B-Cell & Follicular Dendritic Cell Marker); Complement C3d receptor (C3DR); Complement Receptor type 2 (CR2); CVID7; EBV receptor; EBV-R; Epstein-Barr virus receptor; EVBR; SLEB9; anti-CD21 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rCR2/1952
• Specificity: Recognizes a protein of 140kDa, which is identified as the complement receptor 2(CR2)/CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro.CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha.CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA &azide at 1.0mg/ml.
• Sequence Length: 1033

• Applicable Applications for anti-CD21 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: WEHI-231 cells. Human tonsil (IHC).
• Immunogen: Recombinant fragment (around aa 44-196) of human CR2 (CD21) protein (exact sequence is proprietary)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 1q32
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human spleen stained with CD21 Recombinant Mouse Monoclonal Antibody (rCR2/1952).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human spleen stained with CD21 Recombinant Mouse Monoclonal Antibody (rCR2/1952).)

SDS-Page

(SDS-PAGE Analysis Purified CD21 Recombinant Mouse Monoclonal Antibody (rCR2/1952). Confirmation of Integrity and Purity of Antibody.)

References

Otsuka, M., et al. 2004. Role of CD21 antigen in diffuse large B-cell lymphoma and its clinical significance. Br. J. Haematol. 127: 416

NCBI and Uniprot Product Information

• NCBI GI #: 215273962
• NCBI GeneID: 1380
• UniProt Accession #: P20023
• Molecular Weight: 140kDa
• NCBI Official Full Name: Complement receptor type 2
• NCBI Official Synonym Full Names: complement C3d receptor 2
• NCBI Official Symbol: CR2
• NCBI Official Synonym Symbols: CR; C3DR; CD21; CVID7; SLEB9
• NCBI Protein Information: complement receptor type 2
• UniProt Protein Name: Complement receptor type 2
• UniProt Gene Name: CR2
• UniProt Synonym Gene Names: C3DR; Cr2; EBV receptor
• UniProt Entry Name: CR2_HUMAN

NCBI Description

This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

CR2: Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Defects in CR2 are the cause of immunodeficiency, common variable, type 7 (CVID7). A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. Belongs to the receptors of complement activation (RCA) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: plasma membrane; integral to membrane; receptor complex; external side of plasma membrane

Molecular Function: complement binding; protein homodimerization activity; transmembrane receptor activity; complement receptor activity; DNA binding

Biological Process: B cell proliferation; complement receptor mediated signaling pathway; B cell differentiation; innate immune response; immune response; complement activation, classical pathway

Disease: Systemic Lupus Erythematosus, Susceptibility To, 9; Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 7