Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (34BE12).)
Mouse Cytokeratin, Basic Monoclonal Antibody | anti-KRT76 antibody
Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
KRT76; KRT2B; KRT2P; HUMCYT2A
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
Cytokeratin; Basic; Monoclonal Antibody; Basic (Type II or HMW) (Epithelial Marker) Mouse Monoclonal Antibody; KRT2B; KRT2P; HUMCYT2A; Keratin; type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9; anti-KRT76 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
34BE12
Specificity
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
638
Applicable Applications for anti-KRT76 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1.0ug/ml)
Western Blot (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunofluorescence (0.5-1.0ug/ml)
Western Blot (0.5-1.0ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Cytoplasmic
Immunogen
Solubilized keratin extract from human stratum corneum
Hu-Chromosome Location
12q13.13
Positive Control
PC12 cells, Skin, Prostate carcinoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (34BE12).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (34BE12).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (34BE12).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin, HMW Monoclonal Antibody (34BE12).)
References
Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58 Varma M et. al. Mod Pathol 1999;12(5):472-8
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50-67kDa
NCBI Official Full Name
keratin, type II cytoskeletal 2 oral
NCBI Official Synonym Full Names
keratin 76
NCBI Official Symbol
KRT76
NCBI Official Synonym Symbols
KRT2B; KRT2P; HUMCYT2A
NCBI Protein Information
keratin, type II cytoskeletal 2 oral
UniProt Protein Name
Keratin, type II cytoskeletal 2 oral
Protein Family
UniProt Gene Name
KRT76
UniProt Synonym Gene Names
KRT2B; KRT2P; CK-2P; K2P; K76
UniProt Entry Name
K22O_HUMAN
NCBI Description
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq, Jun 2009]
Uniprot Description
K76: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: nucleus
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Product Notes
The KRT76 krt76 (Catalog #AAA438002) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Mouse Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin, Basic can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (0.5-1.0ug/ml) Western Blot (0.5-1.0ug/ml) Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the KRT76 krt76 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin, Basic, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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