Goat SCRIB/Scribble Antibody | anti-SCRIB antibody

Goat Anti-SCRIB/Scribble Antibody

Cat. Num. AAA423847

• Gene Names: SCRIB; CRIB1; SCRB1; SCRIB1; Vartul
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SCRIB/Scribble; Goat Anti-SCRIB/Scribble Antibody; SCRIB; scribbled homolog (Drosophila); CRIB1; SCRB1; SCRIB1; Vartul; scribble; anti-SCRIB antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 1655

• Applicable Applications for anti-SCRIB antibody: Peptide ELISA (EIA), WB, Immunofluorescence (IF)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:1000; WB: Approx 200-250kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178kDa according to NP_874365.3), The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.; IF: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10ug/ml.
• Immunogen: Peptide with sequence C-PEGPGKEKELPGQ, from the internal region of the protein sequence according to NP_874365.3; NP_056171.3.
• Peptide Sequence: PEGPGKEKELPGQ
• Epitope: Internal region
• Important comments: This antibody is expected to recognize both reported isoforms (NP_874365.3; NP_056171.3).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((2ug/ml) staining of HeLa (A), HepG2 (B) and U2OS (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cell junction staining. The nuclear stain is DAPI )

NCBI and Uniprot Product Information

• NCBI GI #: 355390313
• NCBI GeneID: 23513
• NCBI Accession #: NP_874365.3; NP_056171.3
• NCBI GenBank Nucleotide #: NP_874365.3
• UniProt Accession #: Q14160
• NCBI Official Full Name: protein scribble homolog isoform a
• NCBI Official Synonym Full Names: scribble planar cell polarity protein
• NCBI Official Symbol: SCRIB
• NCBI Official Synonym Symbols: CRIB1; SCRB1; SCRIB1; Vartul
• NCBI Protein Information: protein scribble homolog
• UniProt Protein Name: Protein scribble homolog
• Protein Family: Protein
• UniProt Gene Name: SCRIB
• UniProt Synonym Gene Names: CRIB1; KIAA0147; LAP4; SCRB1; VARTUL; Scribble; hScrib
• UniProt Entry Name: SCRIB_HUMAN

NCBI Description

This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011]