LCAT Antibody | anti-LCAT antibody

Goat anti-LCAT (aa366-378) Antibody

Cat. Num. AAA423808

• Reactivity: Human; Expected from sequence similarity: Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: LCAT; Goat anti-LCAT (aa366-378) Antibody; lecithin-cholesterol acyltransferase; phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase; anti-LCAT antibody

• Reactivity: Human; Expected from sequence similarity: Mouse, Rat, Dog, Pig, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 440

• Applicable Applications for anti-LCAT antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:2000.; WB: Approx 55kDa band observed in Human Testis lysates. Recommended concentration: 0.3-1ug/ml.
• Immunogen Sequence: Peptide with sequence C-EDGDDTVATRSTE, from the internal region of the protein sequence according to NP_000220.1.
• Peptide Sequence + C: C-EDGDDTVATRSTE
• Epitope: Internal Region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((1ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4557892
• NCBI GeneID: 3931
• NCBI Accession #: NP_000220.1
• NCBI GenBank Nucleotide #: NM_000229.1
• UniProt Accession #: P04180; Q53XQ3
• Molecular Weight: 49.6kDa
• NCBI Official Full Name: phosphatidylcholine-sterol acyltransferase
• NCBI Official Synonym Full Names: lecithin-cholesterol acyltransferase
• NCBI Official Symbol: LCAT
• NCBI Protein Information: phosphatidylcholine-sterol acyltransferase
• UniProt Protein Name: Phosphatidylcholine-sterol acyltransferase
• UniProt Gene Name: LCAT

NCBI Description

This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:10329423, PubMed:19065001, PubMed:26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:10722751).