Western Blot (WB)
((1ug/ml) staining of Daudi,(A) Jurkat (B) and Jurkat nuclear (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Goat PHF11 Polyclonal Antibody | anti-PHF11 antibody
Goat anti-PHF11 Antibody
Gene Names
PHF11; APY; BCAP; IGEL; IGER; IGHER; NYREN34; NY-REN-34
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PHF11; Polyclonal Antibody; Goat anti-PHF11 Antibody; Goat Anti-PHF11 Antibody; PHD finger protein 11; APY; BCAP; IGEL; IGER; IGHER; NY-REN-34; NYREN34; RP11-185C18.3; IgE responsiveness (atopic); NY-REN-34 antigen; PHF11 antibody; PHD finger protein 11 antibody; APY antibody; BCAP antibody; IGEL antibody; IGER antibody; IGHER antibody; NY-REN-34 antibody; NYREN34 antibody; RP11-185C18.3 antibody; IgE responsiveness (atopic) antibody; NY-REN-34 antigen antibody; anti-PHF11 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KIHASQQRWQQLKE
Sequence Length
331
Applicable Applications for anti-PHF11 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 37kDa band observed in nuclear lysates of cell lines Daudi, Jurkat and Jurkat (calculated MW of 37.6kDa according to NP_001035533.1). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 37kDa band observed in nuclear lysates of cell lines Daudi, Jurkat and Jurkat (calculated MW of 37.6kDa according to NP_001035533.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-KIHASQQRWQQLKE, from the internal region of the protein sequence according to NP_001035533.1; NP_001035534.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_001035533.1; NP_001035534.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Daudi,(A) Jurkat (B) and Jurkat nuclear (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((1ug/ml) staining of Daudi,(A) Jurkat (B) and Jurkat nuclear (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,499 Da
NCBI Official Full Name
PHD finger protein 11 isoform a
NCBI Official Synonym Full Names
PHD finger protein 11
NCBI Official Symbol
PHF11
NCBI Official Synonym Symbols
APY; BCAP; IGEL; IGER; IGHER; NYREN34; NY-REN-34
NCBI Protein Information
PHD finger protein 11
UniProt Protein Name
PHD finger protein 11
Protein Family
UniProt Gene Name
PHF11
UniProt Synonym Gene Names
BCAP
UniProt Entry Name
PHF11_HUMAN
NCBI Description
This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Uniprot Description
PHF11: Positive regulator of Th1-type cytokine gene expression. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 13q14.2
Cellular Component: nucleus
Molecular Function: zinc ion binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
Disease: Asthma, Susceptibility To; Ige Responsiveness, Atopic
Research Articles on PHF11
Similar Products
Product Notes
The PHF11 phf11 (Catalog #AAA423470) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PHF11 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PHF11 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 37kDa band observed in nuclear lysates of cell lines Daudi, Jurkat and Jurkat (calculated MW of 37.6kDa according to NP_001035533.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PHF11 phf11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KIHASQQRWQ QLKE. It is sometimes possible for the material contained within the vial of "PHF11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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