Western Blot (WB)
((1ug/ml) staining of Human lung (A) and Human Umbical cord (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour.)
Goat ECSM2 Polyclonal Antibody | anti-ECSM2 antibody
Goat anti-ECSM2 Antibody
Gene Names
ECSCR; ARIA; ECSM2
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ECSM2; Polyclonal Antibody; Goat anti-ECSM2 Antibody; endothelial cell-specific marker 2; endothelial cell-specific molecule 2; hypothetical protein LOC641700; endothelial cell-specific molecule 2 antibody; hypothetical protein LOC641700 antibody; anti-ECSM2 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
NNGKQSLSAEKVL
Sequence Length
205
Applicable Applications for anti-ECSM2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 50kDa band observed in Human Lung and Human Umbilical Cord lysates (calculated MW of 21.3kDa according to NP_001071161.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ref PMID: 18556573). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 50kDa band observed in Human Lung and Human Umbilical Cord lysates (calculated MW of 21.3kDa according to NP_001071161.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ref PMID: 18556573). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-NNGKQSLSAEKVL, from the C Terminus of the protein sequence according to NP_001071161.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Human lung (A) and Human Umbical cord (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour.)
Western Blot (WB)
((1ug/ml) staining of Human lung (A) and Human Umbical cord (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,295 Da
NCBI Official Full Name
endothelial cell-specific chemotaxis regulator isoform 1
NCBI Official Synonym Full Names
endothelial cell surface expressed chemotaxis and apoptosis regulator
NCBI Official Symbol
ECSCR
NCBI Official Synonym Symbols
ARIA; ECSM2
NCBI Protein Information
endothelial cell-specific chemotaxis regulator
UniProt Protein Name
Endothelial cell-specific chemotaxis regulator
UniProt Gene Name
ECSCR
UniProt Synonym Gene Names
ECSM2; ARIA
UniProt Entry Name
ECSCR_HUMAN
NCBI Description
The protein encoded by this gene is primarily found in endothelial cells and blood vessels, where it is involved in cell shape changes and EGF-induced cell migration. It can enhance the activation of vascular endothelial growth factor receptor-2/kinase insert domain receptor and also promote the proteolysis of internalized kinase insert domain receptor. This gene may play a role in angiogenesis-related diseases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Uniprot Description
ECSM2: Regulates endothelial chemotaxis and tube formation. Has a role in angiogenesis via modulation of the actin cytoskeleton. Belongs to the ECSCR family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q31.2
Cellular Component: cytosol; integral to membrane; plasma membrane
Biological Process: angiogenesis; apoptosis; cell differentiation; chemotaxis; negative regulation of angiogenesis
Research Articles on ECSM2
Similar Products
Product Notes
The ECSM2 ecscr (Catalog #AAA423246) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ECSM2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ECSM2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 50kDa band observed in Human Lung and Human Umbilical Cord lysates (calculated MW of 21.3kDa according to NP_001071161.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ref PMID: 18556573). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ECSM2 ecscr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NNGKQSLSAE KVL. It is sometimes possible for the material contained within the vial of "ECSM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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