Goat MED12 Polyclonal Antibody | anti-MED12 antibody
Goat anti-MED12 (aa1981-1994) Antibody
Western Blot: Preliminary experiments gave an approx 70kDa band in Human Brain (Olfactory Bulb) and Rat Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 243kDa according to NP_005111.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI and Uniprot Product Information
NCBI Description
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]
Uniprot Description
MED12: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: Xq13
Cellular Component: centrosome; membrane; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; ubiquitin ligase complex
Molecular Function: beta-catenin binding; chromatin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein C-terminus binding; protein domain specific binding; receptor activity; thyroid hormone receptor binding; transcription coactivator activity; transcription cofactor activity; vitamin D receptor binding
Biological Process: androgen receptor signaling pathway; embryonic neurocranium morphogenesis; endoderm development; heart development; negative regulation of Wnt receptor signaling pathway; neural tube closure; oligodendrocyte development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein ubiquitination; Schwann cell development; spinal cord development; stem cell maintenance; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway
Disease: Lujan-fryns Syndrome; Ohdo Syndrome, X-linked; Opitz-kaveggia Syndrome
Research Articles on MED12
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Product Notes
The MED12 med12 (Catalog #AAA423100) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MED12 (aa1981-1994) Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MED12 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave an approx 70kDa band in Human Brain (Olfactory Bulb) and Rat Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 243kDa according to NP_005111.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the MED12 med12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NPTLVDPTRH LQQR. It is sometimes possible for the material contained within the vial of "MED12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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