Western Blot (WB)
((0.1ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Cyb5r4 Polyclonal Antibody | anti-Cyb5r4 antibody
Goat anti-Cyb5r4 (mouse aa444-458) Antibody
Gene Names
Cyb5r4; b5b5r; B5+B5R; C79736; Ncb5or; b5/b5r; Cb5cb5r; 2810034J18Rik
Reactivity
Tested: Rat; Expected from sequence similarity: Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Cyb5r4; Polyclonal Antibody; Goat anti-Cyb5r4 (mouse aa444-458) Antibody; cytochrome b5 reductase 4; 2810034J18Rik; B5+B5R; C79736; Cb5cb5r; Ncb5or; b5/b5r; b5b5r; N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein; NADPH cytochrome B5 oxidoreductase; cb5/cb5R; cytochrome b-type NAD(P)H; 2810034J18Rik antibody; b5/b5r antibody; B5+B5R antibody; b5b5r antibody; C79736 antibody; cb5/cb5R antibody; Cb5cb5r antibody; Cyb5r4 antibody; cytochrome b5 reductase 4 antibody; cytochrome b-type NAD(P)H oxidoreductase antibody; flavohemoprotein b5/b5R antibody; NADPH cytochrome B5 oxidoreductase antibody; Ncb5or antibody; N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein antibody; Cyb5r4 (mouse aa444-458); anti-Cyb5r4 antibody
Host
Goat
Reactivity
Tested: Rat; Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RCQLEKLALREKRFD
Sequence Length
528
Applicable Applications for anti-Cyb5r4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 65kDa band observed in Rat Spleen lysates (calculated MW of 55kDa according to NP_596918.2 and 58.8kDa according to Q68EJ0-1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 65kDa band observed in Rat Spleen lysates (calculated MW of 55kDa according to NP_596918.2 and 58.8kDa according to Q68EJ0-1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence RCQLEKLALREKRFD, from the internal region of the protein sequence according to NP_077157.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,994 Da
NCBI Official Full Name
cytochrome b5 reductase 4
NCBI Official Synonym Full Names
cytochrome b5 reductase 4
NCBI Official Symbol
Cyb5r4
NCBI Official Synonym Symbols
b5b5r; B5+B5R; C79736; Ncb5or; b5/b5r; Cb5cb5r; 2810034J18Rik
NCBI Protein Information
cytochrome b5 reductase 4
UniProt Protein Name
Cytochrome b5 reductase 4
Protein Family
UniProt Gene Name
Cyb5r4
UniProt Synonym Gene Names
Ncb5or; b5+b5R
UniProt Entry Name
NB5R4_MOUSE
Uniprot Description
CYB5R4: NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and methemoglobin. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Protein type: Oxidoreductase; Endoplasmic reticulum; EC 1.6.2.2; Cell development/differentiation
Cellular Component: cytoplasm; endoplasmic reticulum; perinuclear region of cytoplasm
Molecular Function: cytochrome-b5 reductase activity; FAD binding; heme binding; metal ion binding; NADPH-hemoprotein reductase activity; oxidoreductase activity; oxidoreductase activity, acting on NADH or NADPH, heme protein as acceptor; protein binding
Biological Process: cell development; generation of precursor metabolites and energy; glucose homeostasis; heme metabolic process; insulin secretion; NADP metabolic process; response to antibiotic; superoxide metabolic process
Research Articles on Cyb5r4
Similar Products
Product Notes
The Cyb5r4 cyb5r4 (Catalog #AAA423021) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Cyb5r4 (mouse aa444-458) Antibody reacts with Tested: Rat; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyb5r4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 65kDa band observed in Rat Spleen lysates (calculated MW of 55kDa according to NP_596918.2 and 58.8kDa according to Q68EJ0-1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the Cyb5r4 cyb5r4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RCQLEKLALR EKRFD. It is sometimes possible for the material contained within the vial of "Cyb5r4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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