Goat LSM5 Polyclonal Antibody | anti-LSM5 antibody

Goat anti-LSM5 Antibody

Cat. Num. AAA422975

• Gene Names: LSM5; YER146W
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: LSM5; Polyclonal Antibody; Goat anti-LSM5 Antibody; LSM5 homolog; U6 small nuclear RNA associated (S. cerevisiae); FLJ12710; YER146W; OTTHUMP00000123495; OTTHUMP00000202663; OTTHUMP00000202664; OTTHUMP00000202665; OTTHUMP00000202666; OTTHUMP00000202667; U6 snRNA-associated Sm-like protein LSm5; FLJ12710 antibody; LSM5 antibody; U6 small nuclear RNA associated (S. cerevisiae) antibody; OTTHUMP00000123495 antibody; OTTHUMP00000202663 antibody; OTTHUMP00000202664 antibody; OTTHUMP00000202665 antibody; OTTHUMP00000202666 antibody; OTTHUMP00000202667 antibody; U6 snRNA-associated Sm-like protein LSm5 antibody; YER146W antibody; anti-LSM5 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KCIGSRIHIVMKSDK
• Sequence Length: 91

• Applicable Applications for anti-LSM5 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 8000.; Western Blot: Preliminary experiments in lysates of cell lines HeLa and MCF7gave no specific signal but low background (at antibody concentration up to 1ug/ml).
• Immunogen: Peptide with sequence KCIGSRIHIVMKSDK, from the internal region of the protein sequence according to NP_036454.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize isoform a (NP_036454.1) only.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 6912488
• NCBI GeneID: 23658
• NCBI Accession #: NP_036454.1
• NCBI GenBank Nucleotide #: NM_012322.2
• Molecular Weight: 6,850 Da
• NCBI Official Full Name: U6 snRNA-associated Sm-like protein LSm5 isoform a
• NCBI Official Synonym Full Names: LSM5 homolog, U6 small nuclear RNA and mRNA degradation associated
• NCBI Official Symbol: LSM5
• NCBI Official Synonym Symbols: YER146W
• NCBI Protein Information: U6 snRNA-associated Sm-like protein LSm5
• UniProt Protein Name: U6 snRNA-associated Sm-like protein LSm5
• Protein Family: Sm-like protein
• UniProt Gene Name: LSM5
• UniProt Entry Name: LSM5_HUMAN

NCBI Description

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM, Apr 2004]

Uniprot Description

LSM5: Plays a role in U6 snRNP assembly and function. Binds to the 3' end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. Belongs to the snRNP Sm proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: cytosol; nucleoplasm; nucleus; spliceosome; U4/U6 x U5 tri-snRNP complex

Molecular Function: protein binding; RNA binding

Biological Process: mRNA processing; nuclear mRNA splicing, via spliceosome