Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PRDM14 Polyclonal Antibody | anti-PRDM14 antibody
Goat anti-PRDM14 (aa533-544) Antibody
Western Blot: Approx 70kDa band observed in lysates of cell line A549 (calculated MW of 64.1kDa according to NP_078780.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]
Uniprot Description
PRDM14: a probable protein lysine methyltransferase that works in concert with the core human embryonic stem cell (ESC) regulators to control pluripotency associated genes. Binds to silenced genes in human ESCs. Its binding profile is enriched for the repressive tri-methylation of histone H3 lysine 27 (H3K27me3) modification. Interacts directly with the polycomb repressive complex 2 (PRC2). PRC2 is detected at PRDM14-bound loci in human ESCs. A probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. Has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.
Protein type: C2H2-type zinc finger protein; Methyltransferase, protein arginine, predicted
Chromosomal Location of Human Ortholog: 8q13.3
Cellular Component: nucleoplasm
Molecular Function: chromatin DNA binding; metal ion binding; methyltransferase activity; protein binding; RNA binding
Biological Process: cell fate specification; cell morphogenesis; embryo implantation; fertilization; germ cell development; germ-line stem cell maintenance; homeostasis of number of cells within a tissue; inner cell mass cell fate commitment; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; somatic stem cell maintenance; transcription, DNA-dependent
Research Articles on PRDM14
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Product Notes
The PRDM14 prdm14 (Catalog #AAA422959) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PRDM14 (aa533-544) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PRDM14 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 70kDa band observed in lysates of cell line A549 (calculated MW of 64.1kDa according to NP_078780.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PRDM14 prdm14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DSHVRRSHKE DD. It is sometimes possible for the material contained within the vial of "PRDM14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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