Goat GPR83 Polyclonal Antibody | anti-GPR83 antibody

Goat anti-GPR83 (aa17-30) Antibody

Cat. Num. AAA422885

• Gene Names: GPR83; GIR; GPR72
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: GPR83; Polyclonal Antibody; Goat anti-GPR83 (aa17-30) Antibody; Goat Anti-GPR83 (aa59-71) Antibody; G protein-coupled receptor 83; GIR; GPR72; G protein-coupled receptor 72; G-protein coupled receptor 72; glucocorticoid induced receptor; probable G-protein coupled receptor 83; G protein-coupled receptor 72 antibody; G protein-coupled receptor 83 antibody; GIR antibody; glucocorticoid induced receptor antibody; GPR72 antibody; G-protein coupled receptor 72 antibody; probable G-protein coupled receptor 83 antibody; GPR83 antibody; GPR83 (aa17-30); anti-GPR83 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: TEPHEGRADEQSAE
• Sequence Length: 423

• Applicable Applications for anti-GPR83 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 2000.; Western Blot: Approx 50kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Amygdala) lysates (calculated MW of 48.3kDa according to NP_057624.3). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence TEPHEGRADEQSAE-C, from the N Terminus of the protein sequence according to NP_057624.3.
• Epitope: N Terminus
• Note: The immunizing peptide represents part of an extracellular domain.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 223633971
• NCBI GeneID: 10888
• NCBI Accession #: NP_057624.3
• NCBI GenBank Nucleotide #: NM_016540.3
• Molecular Weight: 48,339 Da
• NCBI Official Full Name: probable G-protein coupled receptor 83
• NCBI Official Synonym Full Names: G protein-coupled receptor 83
• NCBI Official Symbol: GPR83
• NCBI Official Synonym Symbols: GIR; GPR72
• NCBI Protein Information: probable G-protein coupled receptor 83
• UniProt Protein Name: Probable G-protein coupled receptor 83
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: GPR83
• UniProt Synonym Gene Names: GPR72; KIAA1540
• UniProt Entry Name: GPR83_HUMAN

Uniprot Description

GPR83: Orphan receptor. Could be a neuropeptide Y receptor. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: integral to plasma membrane; nonmotile primary cilium; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide Y receptor activity

Biological Process: cell surface receptor linked signal transduction; neuropeptide signaling pathway; response to glucocorticoid stimulus