Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MTHFR Polyclonal Antibody | anti-MTHFR antibody
Goat anti-MTHFR (aa369-383) Antibody
Western Blot: Approx 70kDa band observed in lysates of cell line NIH3T3 (calculated MW of 74.6kDa according to Human NP_005948.3 and Mouse NP_034970.2). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]
Uniprot Description
MTHFR: Catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co- substrate for homocysteine remethylation to methionine. Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD). MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia, developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders. Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Belongs to the methylenetetrahydrofolate reductase family.
Protein type: Energy Metabolism - methane; EC 1.5.1.20; Oxidoreductase; Cofactor and Vitamin Metabolism - one carbon pool by folate
Chromosomal Location of Human Ortholog: 1p36.3
Cellular Component: cytosol; neuron projection
Molecular Function: FAD binding; methylenetetrahydrofolate reductase (NADPH) activity; NADP binding; protein complex binding
Biological Process: amino acid metabolic process; blood circulation; folic acid metabolic process; homocysteine metabolic process; methionine metabolic process; regulation of histone methylation; response to drug; response to folic acid; response to hypoxia; response to vitamin B2; S-adenosylmethionine metabolic process
Disease: Homocystinuria Due To Deficiency Of N(5,10)-methylenetetrahydrofolate Reductase Activity; Neural Tube Defects, Folate-sensitive; Schizophrenia; Thrombophilia Due To Thrombin Defect
Research Articles on MTHFR
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Product Notes
The MTHFR mthfr (Catalog #AAA422784) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MTHFR (aa369-383) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MTHFR can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 70kDa band observed in lysates of cell line NIH3T3 (calculated MW of 74.6kDa according to Human NP_005948.3 and Mouse NP_034970.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the MTHFR mthfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SRPKSYIYRT QEWDE. It is sometimes possible for the material contained within the vial of "MTHFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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